C omparing the larvicidal activity of phytoecdysteroids present in Spinicia Oleracea subspecies. Done by: Frank Song Jr. Kyra Brower Shi Guan Ming Zhao Xing Liang. Background.
Done by: Frank Song Jr.
Shi Guan Ming
Zhao Xing Liang
Purification of Ecdysteroids:
For further purification, the ecdysteriods are separated by reversed-phase chromatography
In reversed-phase chromatography, octylsilane is used as the stationary phase (being non-polar) instead of silica/alumina.
A cotton wool of mass of 0.2 g was placed at the bottom of the column to prevent alumina from flowing out.
70- 90g (subject to experimental changes) OctylSilane is mixed with the eluent.
To control the flow of the mobile phase, a vacuum will be used at the outlet.