Serum Protein Electrophoresis with Immunofixation. Dr.Ajay Phadke Centre Head SRL Diagnostics- Dr.Avinash Phadke’s Lab. What is electrophoresis?. Electrophoresis is a method of separating proteins based on their physical properties .
SRL Diagnostics-Dr.AvinashPhadke’s Lab
Positive charges of the buffer solution
The Electro-Osmotic Flow (EOF) is a stronger force than the Electrical Field.
As a result, all proteins are carriedtowards the cathodic end of the capillary.
Negative charges of capillarywall
The complementary positively charged ions in the surrounding buffer are free to move under the electromotive force, and they carry with them molecules of the solvent water.
Capillarys Electrophoresis Principle
Capillary in thermo-conductive resin
Easy access to consumables:reagent cups, waste & reagents containers
(2 buffer vials on board)
Gaussian aspect in gamma
No increase or additional deformation/peak
in gamma, beta 1, beta 2 and alpha 2
C3 (and also C4) concentration is a convenient marker for assessing disease activity in rheumatic disorders such as lupus erythematosus and rheumatoid arthritis.
IEP: serum applied to aggelin wells. EP . Antisera added. 24 hr incubation. ARCS formed
Complete substractionwith the antiserumagainst a heavychain and partial substractionwith the antiseraagainst kappa and lambda
Complete substraction of the peakwith one antiserumagainst a heavychain and a light chain
The peak disappears in Kappa
Conclusion: Detection of monoclonal Ig G Kappa
« If removingsomething, whatisremaining? »
In eachwindow, removing one specific class of IgGhighlightswhatishappening with the residualimmunoglobulinsthatremainaftersubstraction
What would you report?
Advise renal profile,UPE and IT
What do you see on the graph. What will you advise?
What is your impression?
What would you advise?
What is your opinion?
What history will you take