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  1. PR1 PR2 PR5 PR4 Pi49 PR10 Fig S2 Gene expression analysis in pot cultures. RNA was extracted from pot cultures and transcript accumulation was analysed by real-time RT-PCR. Shown are mean values and standard deviations obtained with RNA from roots either colonised with F. mosseae (F) with A. euteiches (A) or with both fungi (FA) compared to controls (C). All RNA accumulation values were normalized by the expression of the M. truncatula TEF1 gene. If significant interactions between the factors mycorrhization and pathogen were detected (two-way ANOVA; P = 0.05; n = 3), different letters above the columns indicate significant differences between treatments (Fisher’s LSD; P = 0.05; n = 3).