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Irina V. Kartavtseva, Irina N. Sheremetyeva

GENETICAL FEATURES OF CHROMOSOMAL FORM « C » OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST. Irina V. Kartavtseva, Irina N. Sheremetyeva Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia) Anastasia B. Ryabkova

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Irina V. Kartavtseva, Irina N. Sheremetyeva

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  1. GENETICAL FEATURES OF CHROMOSOMAL FORM«C» OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST Irina V. Kartavtseva, Irina N. Sheremetyeva Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia) Anastasia B. Ryabkova Khabarovsk Antiplague Station, (Russia) Svetlana A. Romanenko, Olga L. Gladkikh Institute of Molecular and Cellular Biology SB RAS (Russia) Lubov V. Frisman Institute of Complex Analysis of Regional Problems Far Eastern Branch of Russian Academy of Sciences, Birobidzhan (Russia)

  2. The role of chromosomal rearrangements in speciation still unclearly. Introduction The maximowicz's vole Microtus maximowiczii (Rodentia) is convenient species for investigating this problem.

  3. All rearrangements for this species can be divided into three types: • Rearrangements by Mejer et al., 1996 1 - is fusion of two middle sized pairs of metacentrics to formating of large metacentric. 2 - is Robertsonian rearrangements. 3 - is pericentric inversions of 4 pairs.

  4. Areal and terra typica points of M. maximowiczii Russia M. m. maximowicziiSchrenck 1858, Amurskii and Khabarovskii regions in Russia, and in northeastern China M. m. ungurensisKastchenko 1913, Buryatia and Chitinskii regions in Russia and North-Eastern Mongolia Mongolia China Korea

  5. For this species was described five chromosomal forms (Kartavtseva et al., 2008): • A : 2n = 39 - 44, NF = 53 - 60 (Buryatiya), • B: 2n = 38 - 42, NF = 54 - 57 (Buryatiya), • C: 2n = 39 - 41, NF = 55 - 60 (Far East) • D: 2n= 40=43, NF= 54-58 (Zabaikalskii kray and E. Mongolia) • V: 2n = 36 - 40, NF = 52 - 56 (Transbaikalia and W.Mongolia).

  6. 5 chromosomal forms of M. maximowiczii (Kartavtseva et al., 2008) «B» 2n=38-42 «A» 2n=39-44 Russia M. m. ungurensis M. m. maximowiczii Mongolia «D» 2n=40-43 «C» 2n=39-41 «V» 2n=40-43 China

  7. "A“ 2n=39-44 M. m. ungurensis M. m. maximowiczii "C“ 2n=39-41

  8. control region mtDNA Mmax45 83 Mmax41 78 Mmax38 45 Mmax39 98 42 Mmax42 68 Mmax40 Mmax37 55 Mmax43 Mmax44 65 25 Mmax47 45 Mmax33 79 Mmax48 Mmax51 78 Mmax52 74 Mmax49 59 52 Mmax50 34 Mmax33 93 65 Mmax34 62 Mmax36 Mmax35 Mmax8 80 96 29 Mmax11 Mmax7 50 Mmax9 50 Mmax10 92 Mmax30 Mmax29 74 95 Mmax25 Mmax26 73 Mmax27 69 Mmax31 50 Mmax28 47 Mmax32 31 Mmax1 68 Mmax2 51 Mmax4 Mmax5 87 Mmax3 83 Mmax6 Mmax16 98 Mmax17 76 76 Mmax18 Mmax24 Mmax14 Mmax22 55 Mmax13 Mmax12 Mmax15 98 Mmax23 Mmax21 Mmax19 13 Mmax20 Microtus oeconomus 0.01 M. m. maximowiczii M. m. ungurensis С “Восточная” А D “Южная” B “Западная” V

  9. MaterialMap of chromosomal investigation We investigated karyotype of 36 individuals of Maximowicz's vole from six earlier not investigated populations of the territory Middle Amur river (chromosomal form C). Literature data: • 2n=42 • 2n=39 Our data: • (2n=40-41) • New data

  10. 2n=40-41 2n=41 2n=40

  11. Chromosomal forms “D” and “C” 2n=42 2n=40

  12. New chromosomal nomenclature of M. maximowiczii by fluorescent in situ hybridization (FISH) 20 M. maximowiczii (#2350) from Romanovka (chromosomal form “A”, our data) 11,20 11 2n=41 M. maximowiczii FISH by Lemskaja et al., 2010 from chromosomal form “A”

  13. M. Maximowiczii (#2060)from Norskii reservation (our data) 11 20 20 11

  14. Map of investigation of control region A Bmt DNA 940 np • A A B

  15. Conclusion In the territory Middle Amur river (chromosomal form C): • Stabilization of karyotypewith Robertsonian rearrangement chromosomal pairs 11 and 20 and inversion of pair 14 • Two clasters of control region of mt DNA can be results of isolations of two animals groups.

  16. Thanks for attention

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