DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems
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DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems . Mark Bagley, United States Environmental Protection Agency Charles Spooner, US EPA Ronald Klauda Maryland Dept of Natural Resources David Schindel, Consortium for the Barcode of Life

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DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems

Mark Bagley, United States Environmental Protection Agency

Charles Spooner, US EPA

Ronald Klauda Maryland Dept of Natural Resources

David Schindel, Consortium for the Barcode of Life

Lee Weigt, Smithsonian Institution

Robert Hanner, University of Guelph


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Ecological Integrity Aquatic Ecosystems

Physical

Integrity

Chemical

Integrity

Biological

Integrity

Bioassessment

An evaluation of the

biological condition of a

waterbody using biological

surveys and other direct

measurements of the resident living organisms


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US Wadeable Streams Assessment Aquatic Ecosystems

  • National assessment of the condition of wadeable streams

  • 10 different taxonomic ID laboratories

  • 749 stream macroinvertebrate samples (sites)

  • All organisms identified to genus only

  • 10% random re-identification by independent taxonomist

  • Data quality objective – 85% repeatability

Credible environmental decision-making depends on objectivity and repeatability of taxonomic results


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EPA Advanced Monitoring Initiative Aquatic Ecosystems

Project Goals

  • Develop a DNA barcode library for important aquatic indicator species (EPT)

    • Ephemeroptera (Mayflies)

    • Plecoptera (Stoneflies)

    • Trichoptera (Caddisflies)

  • Compare DNA barcodes to traditional bioassessments for EPT taxa

    • Cost, Speed, Objectivity, Accuracy, Precision

    • How important is increased taxonomic precision?

  • Determine how to efficiently incorporate DNA barcodes into a state bioassessment program


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Repeatability and barcode development Aquatic Ecosystems

Reference

Barcode

Database

Maryland

DNR

Guelph

(taxonomic

agreement)

Smithsonian

(Adult

Voucher

specimens)

EPA

Lab

EPA

(disagreement

or MOTU)

Taxon Experts

Morphology

DNA

Repeatability

Accuracy

Precision

Cost

Repeatability

Precision

Cost

Species

Description


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Tech Transfer is a Major Project Goal Aquatic Ecosystems

  • End users are participants in the project

    • Maryland DNR, EPA-Water

  • Tech transfer documents, hands-on workshops, and protocols are key products

  • Chose influential end-users that will “convert” others