IRES vs 2A peptide
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IRES vs 2A peptide. GFP IRES mCherry. mCherry. R. U5. R. U5. eGFP. D U3. D U3. RRE. cPPT. IRES. WPRE. SFFV. GFP 2A mCherry. mCherry. R. U5. R. U5. eGFP. D U3. D U3. RRE. cPPT. WPRE. SFFV. 2A. 0.31. 12.7. 0.22. 14.4. 5. 5. 10. 10. 4. 4. 10. 10. 3. 3. 10.

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Gfp ires mcherry

IRES vs 2A peptide

GFP IRES mCherry

mCherry

R

U5

R

U5

eGFP

DU3

DU3

RRE

cPPT

IRES

WPRE

SFFV

GFP 2A mCherry

mCherry

R

U5

R

U5

eGFP

DU3

DU3

RRE

cPPT

WPRE

SFFV

2A


Gfp ires mcherry

0.31

12.7

0.22

14.4

5

5

10

10

4

4

10

10

3

3

10

10

mCherry

mCherry

2

2

10

10

0

0

0.53

82.7

2.71

86.5

2

3

4

5

2

3

4

5

0

10

10

10

10

0

10

10

10

10

GFP

GFP

Day8 post-transduction in Nalm6 cells

GFP IRES mCherry

GFP 2A mCherry

16% GFP without mCherry

4% GFP without mCherry


Gfp ires mcherry

5

10

4

10

Texas Red PE-A

3

10

2

10

0

2

3

4

5

0

10

10

10

10

GFP-A

GFP IRES mCherry

GFP 2A mCherry

Overlay of FACS plots