1 / 3

GFP IRES mCherry

IRES vs 2A peptide. GFP IRES mCherry. mCherry. R. U5. R. U5. eGFP. D U3. D U3. RRE. cPPT. IRES. WPRE. SFFV. GFP 2A mCherry. mCherry. R. U5. R. U5. eGFP. D U3. D U3. RRE. cPPT. WPRE. SFFV. 2A. 0.31. 12.7. 0.22. 14.4. 5. 5. 10. 10. 4. 4. 10. 10. 3. 3. 10.

cairo-booker
Download Presentation

GFP IRES mCherry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. IRES vs 2A peptide GFP IRES mCherry mCherry R U5 R U5 eGFP DU3 DU3 RRE cPPT IRES WPRE SFFV GFP 2A mCherry mCherry R U5 R U5 eGFP DU3 DU3 RRE cPPT WPRE SFFV 2A

  2. 0.31 12.7 0.22 14.4 5 5 10 10 4 4 10 10 3 3 10 10 mCherry mCherry 2 2 10 10 0 0 0.53 82.7 2.71 86.5 2 3 4 5 2 3 4 5 0 10 10 10 10 0 10 10 10 10 GFP GFP Day8 post-transduction in Nalm6 cells GFP IRES mCherry GFP 2A mCherry 16% GFP without mCherry 4% GFP without mCherry

  3. 5 10 4 10 Texas Red PE-A 3 10 2 10 0 2 3 4 5 0 10 10 10 10 GFP-A GFP IRES mCherry GFP 2A mCherry Overlay of FACS plots

More Related