Yin yang column for global protein identification
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Yin-Yang Column for Global Protein Identification. Column Technology Inc. The Yin-Yang column combines anion and cation exchange chromatography For the protein identification through multi-LC/MS/MS:. Yin-Yang MDLC-MS/MS. Advantages of Yin-yang MDLC-MS/MS. Three-dimensional LC-MS/MS

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Presentation Transcript

The Yin-Yang column combines anion and cation

exchange chromatography

For the protein identification through multi-LC/MS/MS:

Yin-Yang MDLC-MS/MS


Advantages of yin yang mdlc ms ms
Advantages of Yin-yang MDLC-MS/MS

  • Three-dimensional LC-MS/MS

  • SCX and ANX are complimentary methods for peptide fractionation

  • Most complete coverage of peptides and proteins

  • Enrichment and high-sensitivity for phosphopeptides

  • Identify phospho and non-phospho peptides in the same run.


Theoretical pI distribution of identified non-phosphrylated peptides

Complementary peptide identification of SCX and SAX


108413 Unique peptides peptides

SAX

SCX

3047

9094

10254

6184 Proteins

SCX

SAX

2040

4152

4072

only 1mg protein digests of mouse liver, 5% FPR

Another More than 1000 phosphopeptides identified


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