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Foodborne Pathogen and Disease. Foodborne Pathogens. a biological infectious agent ( Microorganism ) that causes Foodborne illness to host (referred to as food poisoning ) is any illness resulting from the consumption of contaminated food. . Foodborne Pathogens :.

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Foodborne Pathogen and Disease

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foodborne pathogens
Foodborne Pathogens

a biologicalinfectious agent (Microorganism) that causes Foodborne illnessto host(referred to as food poisoning) is any illness resulting from the consumption of contaminated food.

foodborne pathogens3
Foodborne Pathogens:
  • Foodborne pathogens are the leading causes of illness and death in less developed countries killing approximately 1.8 million people annually.
  • In developed countries foodborne pathogens are responsible for millions of cases of infectious gastrointestinal diseases each year, costing billions of dollars in medical care and lost productivity.
New foodborne pathogens and foodborne diseases are likely to emerge driven by factors such as pathogen evolution, changes in agricultural and food manufacturing practices, and changes to the human host status.
  • There are growing concerns that terrorists could use pathogens to contaminate food and water supplies in attempts to incapacitate thousands of people and disrupt economic growth.
pathogenic microorganisms
Pathogenic microorganisms

1. Intoxication :

- Staphylococcus aureus

- Clostridium botulinum

- Bacillus cereus

2. Infection :

- Salmonella

- Clostridium perfringens

- Vibrio

- Phathogenic E.coli

staphylococcus aureus


  • Gram positive coccus (producing an exotoxin)

Source of contamination of food

- nose and skin of humans and animals

- high level in people with

- skin infection

- heavily colonised skin disease

Contamination by food handler
  • Eliminated by pasteurization
  • Microwave decreases counts
  • Fat/ sugar/ salt protects the organism
  • Usually about 106 / g needed to produce sufficient toxin
  • Generally low numbers are allowed in food
growth requirements

* Temperature range

7 - 48 o C (optimum 37 oC)

* pH range

4 - 9.8 (optimum 6 - 7)

* Facultative anaerobe

requirements for toxin production
  • Range is more limited than growth range

optimum temperature 40 – 45 o C

Aw above 0.85

staphylococcal exotoxin enterotoxin neurotoxin
Staphylococcal Exotoxin(Enterotoxin / Neurotoxin)

* Resistant to proteolytic enzymes

e.g. trypsin in the gut

* Resistant to heat

mechanism of activity
Mechanism of Activity
  • Toxin (exotoxin) is performed and ingested in food
  • Stimulates neural receptors in the gastrointestinal tract
  • Vomiting within approx 4 hours (1-6 hours) after ingestion of toxin
  • The toxin can also induce diarrhea, nausea, headache
examples of foods implicated in outbreaks
Examples of foods implicated in outbreaks
  • Salted meats
  • Cold cooked meats
  • Poultry
  • Custard
  • Cream filled bakery products

(whipped cream)

  • Mayonnaise
  • egg


  • Inadequate in refrigeration
  • Food prepared in advance
  • Poor personal hygiene
  • Moderate cooking or heat processing
  • Holding food in warmer


* Control post-process contamination

* Control temperature abuse (cooking/ holding/ refrigeration)

* Handle food correctly

* Good quality raw material



  • Enterobacteriaceae
  • Gram Negative, Short Rod
  • Non spore forming
  • Peritrichous flagella


  • Intestinal of domestic and wild animal
  • Water, Sewage, Environment
growth requirements15

* Temperature range

optimum 37 oC

42 oCused for selective enrichment

* pH range

4 - 9

* Facultative anaerobe

found in many foods
Found in many foods :
  • Contamination directly or indirectly with animal or human feaces

- Raw/Undercooked eggs

- Uncooked meat

- Raw milk and milk products

- Poultry and poultry products

- Skim milk powder

- Ice-cream

- Mayonnaise

- Chocolate

- Cantaloupes

  • Diarrhoea
  • Vomiting
  • Antibiotics minimal effect
  • Organism may be excreted for weeks
  • Some outbrakes have shown very low infective dose causing death in infants, elderly and immunosuppressed.
  • Correct food hygiene – direct or indirect feacal contamination
  • Correct food processing – heating / cooling / storing.
  • Correct personal hygiene to control secondary spread
  • Food handlers should have consecutive negative feacal cultures before returning to work with food.
  • is the name of a test for the Enterobacteriaceae family.
  • commonly-used bacterial indicator of sanitary quality of foods and water.
  • They are defined as rod-shaped Gram-negative non-spore forming organisms.
  • Some genus can fermentlactose with the production of acid and gas when incubated at 35-37°C.
  • abundant in the feces of warm-blooded animals, but can also be found in the aquatic environment, in soil and on vegetation.
  • they are easy to culture and their presence is used to indicate that other pathogenic organisms of fecal origin may be present. Fecal pathogens include bacteria, viruses,or protozoa and many multicellular parasites
escherichia coli
Escherichia coli
  • Member of the coliform group
  • Gram Negative, rodand Non-sporulating
  • Facultative anaerobic
  • ferment lactose at 44°C in the fecal coliform test
  • When cultured on an EMB plate,

a positive result for E. coli is Metallic

sheen colonies on a dark purple media.


Adams, M.R. and Moss, M.O., Food Microbiology, 2008