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PTT303 /2 PROCESS MODELLING AND SIMULATION SEM 1 ( 2013/2014). Biochemical Case Study. Student should be able to; .
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PROCESS MODELLING AND SIMULATION
SEM 1 (2013/2014)
Biochemical Case Study
moderate complex flowsheet
Sequential modular approach
Water, microorganisms, nutrients (glucose) and air are fed into a bioreactor where at 37°C a fermentation takes place, yielding an enzyme and impurities. Biomass is separated in a disk-stack centrifuge and the liquid is stored in tank. It is then processed in a diafilter where the remaining biomass is removed (with a small loss of product). It is stored again and then loaded onto a PBA chromatography column where the enzyme binds and eluted using a WFI/NaCl mixture.
separated in a disk-stack
centrifuge and the liquid is stored in tank. (storage 1)
Purification Section 2
Initialising CHARGE operation
(right click on unit procedure (Fermentation)then click add /remove operations..
Add Charge -1, Charge-2, Heat-1, Ferment-1, Transfer-Out-1.
Note: Leave other values as DEFAULT
Final temp: 37 °C
Process time: 36 hr
Auto adjust for air
flowsheet & solve
the error message given
Right click on storage; then click on add/remove operations/delete storage/add TRANSFER-OUT-1
Please delete “STORE-1”
operation in P-3 & replace
it with a “Transfer-Out-1”
(Note; Right click on equipment & select “Flip (reverse direction)” to turn the equipment into reverse direction
goes directly to continuous diafiltration
Set TRANSFER-OUT-1 of
Storage1 (P-3) to follow the
duration of Filtration inDiafilter
(P-4) using Master-Slave
Again, replace “STORE
-1” operation in P-5 with
(Note: Right click on equipment & select “Flip (reverse
direction)” to turn the equipment into reverse direction
‘Register as NaCl (2M) & replace the water compound in this mixture with WFI’
Register it from scratch & fill in the physical properties that you have
Make sure the
mass % is make
up into 100%
Highlight the water
component, delete &
replace it with WFI
Create new mixture
Choose this option if
you know the density
of the mixture
Choose this option to modify from an existing mixture (e.g. NaCl mixture)
(Always remember to save your work …)
4 different PBA Chromatography
1)Column Loading (Load)
2)Column Washing (Wash)
3)Column Elution (Elute)
4)Column Regeneration (Regenerate)
Prepare a detail report of the BIOCHEMICAL CASE STUDY and attached together
your simulation result (Gantt Chart)