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S17834 ( M, 1h) 0 2.5 5 10 20 25

Zang, et al. Supplemental Figure 1. A. S17834 ( M, 1h) 0 2.5 5 10 20 25. pAMPK AMPK2 pACC. B.

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S17834 ( M, 1h) 0 2.5 5 10 20 25

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  1. Zang, et al. Supplemental Figure 1 A S17834 (M, 1h) 0 2.5 5 10 20 25 pAMPK AMPK2 pACC B Supplemental Fig. 1. S17834 increases phosphorylation of AMPK  and ACC in a concentration-dependent manner in cultured HepG2 cells. A. Serum-starved HepG2 cells were exposed to increasing concentrations of S17834 (2.5–25 M) or vehicle (DMSO) for 1 h. The phosphorylation of AMPK and ACC as well as the expression of total AMPK2 protein were immunoblotted. B.Phosphorylation levels of AMPK and ACC are expressed as fold stimulation over the basal phosphorylation level, mean  S.E. (n=3). *P < 0.05 compared with vehicle control.

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