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Gene Silencing and Profiling of Transcription Factors Study

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This study uses siRNA to target specific genes in order to study transcription factor activities. Gene silencing impacts are analyzed, providing insights into regulatory mechanisms of key transcription factors.

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Gene Silencing and Profiling of Transcription Factors Study

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  1. Fig. S6. (A) (B) siTIG3(16+5A) S 5’ UCUCAGGCGUUCUCUA AS 3’(dTdT)GACAGAGUCCGCAAGAGAU siSurvivin(16+5A) S 5’ GAGAUCAACAUUUUCA AS 3’(dTdT)UUCCUCUAGUUGUAAAAGU siCalcineurin(16+5A) S 5’ AUGAAUAUUCGACAGU AS 3’(dTdT)CACUACUUAUAAGCUGUCA siATF6(16+5A) S 5’ GCCUCCUCAAGUUAUU AS 3’(dTdT)GGUCGGAGGAGUUCAAUAA siDBP(16+5A) S 5’ AAGACAUCGCUUCUCA AS 3’(dTdT)AGCUUCUGUAGCGAAGAGU siTEF(16+5A) S 5’ GACGCAAGAAGAACAA AS 3’(dTdT)GUUCUGCGUUCUUCUUGUU siHIF-1α-01(16+5A) S 5’ ACUGCAGGGUGAAGAA AS 3’(dTdT)GGAUGACGUCCCACUUCUU siHIF-1α-02(16+5A) S 5’ UAAAGAACAAAACACA AS 3’(dTdT)CCCAUUUCUUGUUUUGUGU  siNF-κB(16+5A) S 5’ CUAUCCCUUUACGUCA AS 3’(dTdT)CGGGAUAGGGAAAUGCAGU siTIG3(19+2) S 5’ CUGUCUCAGGCGUUCUCUA(dTdT) AS 3’(dTdT)GACAGAGUCCGCAAGAGAU siSurvivin(19+2) S 5’ AAGGAGAUCAACAUUUUCA(dTdT) AS3’(dTdT)UUCCUCUAGUUGUAAAAGU siCalcineurin(19+2) S 5’ GUGAUGAAUAUUCGACAGU(dTdT) AS 3’(dTdT)CACUACUUAUAAGCUGUCA siATF6(19+2) S 5’ CCAGCCUCCUCAAGUUAUU(dTdT) AS 3’(dTdT)GGUCGGAGGAGUUCAAUAA siDBP(19+2) S 5’ UCGAAGACAUCGCUUCUCA(dTdT) AS 3’(dTdT)AGCUUCUGUAGCGAAGAGU siTEF(19+2) S 5’ CAAGACGCAAGAAGAACAA(dTdT) AS 3’(dTdT)GUUCUGCGUUCUUCUUGUU siHIF-1α-01(19+2) S 5’ CCUACUGCAGGGUGAAGAA(dTdT) AS 3’(dTdT)GGAUGACGUCCCACUUCUU siHIF-1α-02(19+2) S 5’ GGGUAAAGAACAAAACACA(dTdT) AS 3’(dTdT)CCCAUUUCUUGUUUUGUGU  siNF-κB(19+2) S 5’ GCCCUAUCCCUUUACGUCA(dTdT) AS 3’(dTdT)CGGGAUAGGGAAAUGCAGU

  2. (E) (D) (C) (H) (F) (G) (I) (J) (K)

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