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Gene Silencing and Profiling of Transcription Factors Study

This study uses siRNA to target specific genes in order to study transcription factor activities. Gene silencing impacts are analyzed, providing insights into regulatory mechanisms of key transcription factors.

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Gene Silencing and Profiling of Transcription Factors Study

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  1. Fig. S6. (A) (B) siTIG3(16+5A) S 5’ UCUCAGGCGUUCUCUA AS 3’(dTdT)GACAGAGUCCGCAAGAGAU siSurvivin(16+5A) S 5’ GAGAUCAACAUUUUCA AS 3’(dTdT)UUCCUCUAGUUGUAAAAGU siCalcineurin(16+5A) S 5’ AUGAAUAUUCGACAGU AS 3’(dTdT)CACUACUUAUAAGCUGUCA siATF6(16+5A) S 5’ GCCUCCUCAAGUUAUU AS 3’(dTdT)GGUCGGAGGAGUUCAAUAA siDBP(16+5A) S 5’ AAGACAUCGCUUCUCA AS 3’(dTdT)AGCUUCUGUAGCGAAGAGU siTEF(16+5A) S 5’ GACGCAAGAAGAACAA AS 3’(dTdT)GUUCUGCGUUCUUCUUGUU siHIF-1α-01(16+5A) S 5’ ACUGCAGGGUGAAGAA AS 3’(dTdT)GGAUGACGUCCCACUUCUU siHIF-1α-02(16+5A) S 5’ UAAAGAACAAAACACA AS 3’(dTdT)CCCAUUUCUUGUUUUGUGU  siNF-κB(16+5A) S 5’ CUAUCCCUUUACGUCA AS 3’(dTdT)CGGGAUAGGGAAAUGCAGU siTIG3(19+2) S 5’ CUGUCUCAGGCGUUCUCUA(dTdT) AS 3’(dTdT)GACAGAGUCCGCAAGAGAU siSurvivin(19+2) S 5’ AAGGAGAUCAACAUUUUCA(dTdT) AS3’(dTdT)UUCCUCUAGUUGUAAAAGU siCalcineurin(19+2) S 5’ GUGAUGAAUAUUCGACAGU(dTdT) AS 3’(dTdT)CACUACUUAUAAGCUGUCA siATF6(19+2) S 5’ CCAGCCUCCUCAAGUUAUU(dTdT) AS 3’(dTdT)GGUCGGAGGAGUUCAAUAA siDBP(19+2) S 5’ UCGAAGACAUCGCUUCUCA(dTdT) AS 3’(dTdT)AGCUUCUGUAGCGAAGAGU siTEF(19+2) S 5’ CAAGACGCAAGAAGAACAA(dTdT) AS 3’(dTdT)GUUCUGCGUUCUUCUUGUU siHIF-1α-01(19+2) S 5’ CCUACUGCAGGGUGAAGAA(dTdT) AS 3’(dTdT)GGAUGACGUCCCACUUCUU siHIF-1α-02(19+2) S 5’ GGGUAAAGAACAAAACACA(dTdT) AS 3’(dTdT)CCCAUUUCUUGUUUUGUGU  siNF-κB(19+2) S 5’ GCCCUAUCCCUUUACGUCA(dTdT) AS 3’(dTdT)CGGGAUAGGGAAAUGCAGU

  2. (E) (D) (C) (H) (F) (G) (I) (J) (K)

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