Principles of Fluorescence Spectroscopy. Chemistry Department XMU. Chapter Eight. Measurement of Fluorescence Anisotropy. Content. 8.1 Measurement of Anisotropy 8.2 Polarization Spectra of Fluorophores 8.3 Perrin Equation 8.4 Time-Dependent Decays of Anisotropy
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In vitrified solutions, the measured anisotropy values provide a measure of the angle between the absorption and emission dipoles.
The orientation of the absorption dipole differs for each absorption band and thus the angle varies with wavelength.
Anisotropy varies with the excitation wavelength.