Root Deep Absolute Advance III - Clinical Backbone

1. Clinical Backbone

2. 10 Actions against all 5 concerns of hair fall and decreased hair density / growth on all 3parts of hair. The Everything (Hair) Therapy Fall Reduction Root Energizing Blockage Clearing 4 Nourishing oils Anti Scalp Ageing Dandruff Removal 10 Phytonutrient extracts Shaft Strengthening Volumizing Instant Shine Smoothening Frizz Control 17 Keratin resembling plant amino acids

4. ++++ Proven Hair Fall reduction

5. +++ +++ Pisumsativum sprout extract Study design I • Study period of 12 weeks used twice daily • 20 volunteers of age 21-37 • 17 females with hair in telogen phase > 15% • 3 males with hair in telogen phase > 20% Incorporated in Root Deep Hydroil

6. Effect on hair fall +++ Results

7. Reduction of Hair fall +++ Results

8. Effect on A/T ratio +++ Results

9. Response from volunteers +++ Results

10. +++ +++ Pisumsativum sprout extract Study design II • Study period of 14 days used twice daily • 10 volunteers of age 46-60 • 4 females • 6 males Incorporated in Root Deep Hydroil

11. Effect on the expression of dermal papilla signaling molecules +++ Results • Noggin – A protein that shortens the telogen phase • FGF-7 - Fibroblast growth factor – 7 that initiates new anagen phase

12. +++ +++ Ocimumbasilicum hairy root culture extract Biotechnologically derived, holy plant extract with Anti 5-alpha reductase activity. Provides nourishment to starving hair roots & promotes fuller, denser hair. 5α reductase is the key target enzyme in the inhibition of hair loss. Basil root Culture extract inhibit 5α reductase. Testosterone will not convert into dihydrotestosterone (DHT) in absence of 5α reductase. The results showed a clear concentration dependent inhibition of 5α reductase. Consequently, Basil root Culture extract was able to reduce hair loss in people with androgenic alopecia. Incorporated in Root Deep Hydroil

13. Increased proliferation of Dermal papilla cells +++ Results

14. +++ +++ Ocimumbasilicum hairy root culture extract Study design • Study period of 2 months used once daily • 21 volunteers of age 25-67 with daily hair loss > 100 strains • 19 females • 2 males Incorporated in Root Deep Hydroil

15. +++ +++ Results

16. +++ +++ Pterocarpusmarsupium bark extract Study design • Study period of 6 months used once daily against placebo • 40 male volunteers of with androgenic alopecia Incorporated in Root Deep Hydroil Root Deep Shampoo

17. +++ +++ Results

18. ++++ Proven Root Energizing

19. +++ +++ Plant extracts with Hexapeptides-11 • Plant extracts like sugar cane extract, Lemon fruit extract, Apple fruit extract, green tea leaf extract along with Hexapetides-11 restores hair and scalp vitality at cellular level and energizing hair bulb Incorporated in ElementiPuro Root Recharge Synergy

20. +++ +++ Plant extracts with Hexapeptides-11 Study design • Study period of 5 days used once daily • 15 volunteers of age 30-60 with hair length of not less than 12 inches

21. +++ +++ Results

22. +++ +++ Results

23. +++ +++ Vanillyl Butyl Ether • Enhances microcirculation of blood flow with lesser irritation and warming sensation Incorporated in ElementiPuro Root Recharge Synergy

24. Increase in Blood flow +++ Results

25. Effect on the production of IGF-1 +++ Results

26. ++++ Proven Blockage Clearing

27. +++ +++ Ocimumbasilicum hairy root culture extract Biotechnologically derived, holy plant extract with Anti 5-alpha reductase activity. Provides nourishment to starving hair roots & promotes fuller, denser hair. 5α reductase is the key target enzyme in the inhibition of hair loss. Basil root Culture extract inhibit 5α reductase. Testosterone will not convert into dihydrotestosterone (DHT) in absence of 5α reductase. The results showed a clear concentration dependent inhibition of 5α reductase. Consequently, Basil root Culture extract was able to reduce hair loss in people with androgenic alopecia. Incorporated in Root Deep Hydroil

28. +++ +++ Results Inhibition of 5α reductase

29. +++ +++ Zinc PCA • Zinc salt of pyrrolidone carboxylic acid, a double acting complex that optimally allies the beneficial effects of zinc and L-PCA, physiological components of the organism. Incorporated in ElementiPuro Root Recharge Synergy

30. +++ +++ Zinc PCA

31. +++ +++ Zinc PCA Study design • Inhibition of 5α reductase • Pre-incubation of human Dermal fibroblasts in presence of Zinc PCA / Zinc chloride and Addition of radioactive (titrated) Testosterone and incubation for 24 hours / preparation of cellHomogenates by sonication and Radioactivity measurement • Anti 5-alpha reductase activity of Zinc PCA (+65%) much higher than Zinc chloride (+45%) • Intrinsic activity of Zinc PCA greater than equimolar quantity of Zinc chloride.

32. +++ +++ Results Inhibition of 5α reductase

33. +++ +++ Zinc PCA Study design • Seboregulatory activity • Sebometric study on 2 groups of 16 volunteers after 28 daily application on the half forehead with a cream containing 1% Zinc PCA vs Placebo and non-treated zone Results

34. +++ +++ Zinc PCA Study design • Antimicrobial activity • Minimum Inhibitory Concentration of Zinc PCA was identified and optimized for various bacterial and fungal strains. Results

35. ++++ Proven Anti scalp Aging

36. +++ +++ Pterocarpusmarsupium bark extract Study design • Anti glycation activity • Against aminoguanidine as positive control Incorporated in Root Deep Hydroil Root Deep Shampoo Results

37. +++ +++ Results • Anti free radical activity

38. +++ +++ Pterocarpusmarsupium bark extract Anti-inflammatory effect: Study design I • Test on Keratinocytes: • On the model of UVB induced inflammation against positive control of Aspirin • Incubation period of 72 hours Results

39. +++ +++ Pterocarpusmarsupium bark extract Anti-inflammatory effect: Study design II • Test on Polymorphonuclearneutrophilic granulocytes (PMN): • On the model of UVB induced inflammation against positive control of Minocyclin • Incubation period of 24 hours Results

40. ++++ Proven Dandruff Removal

41. +++ +++ Piper nigrum fruit extract and Inga alba bark extract Anti microbial activity: Study design • Study period of 6 months used once daily against placebo • Against Bacterial strains Malasseziaglobosa and Malasseziafurfur, responsible for dandruff, inflammation and irritation • Incubation period of 48 hours Incorporated in Root Deep Hydroil Root Deep Shampoo Root Deep Intenso Fluid Results

42. +++ +++ Piper nigrum fruit extract and Inga alba bark extract Anti inflammatory activity: Study design • On human keratinocytes on the model of UVB induced inflammation against positive control of Aspirin • Incubation period of 48 hours Results

43. +++ +++ Piper nigrum fruit extract and Inga alba bark extract Improvement of Scalp properties: Study design • To evaluate the scalp conditions (dandruff, sebum) against a shampoo with 1% zinc pyrithione, after 4 weeks of treatment and 2 weeks of remanence period

44. +++ +++ Results Reduction in Dandruff:

45. +++ +++ Results Reduction in Sebum:

46. +++ +++ Results Response of Volunteers:

47. ++++ Proven Shaft strengthening

48. +++ +++ Hydrolyzed Pea Protein • 17 keratin resembling plant amino acids • Improves hair strength lost by damages • Restores hair moisture and prevents dry hair • Smoothens hair surface Incorporated in Root Deep Shampoo Root Deep Intenso Fluid

49. +++ +++ Results Tensile strength measurement:

50. +++ +++ Results Cross-section measurement: