1 / 13

Use of Bacteria in Antibody Production

Use of Bacteria in Antibody Production. Robert Gallo. A big Problem…. There are many diseases in the world caused by pathogens that devastate many people each year, causing pain, financial loss, and death. A Solution….

brett-christensen
Download Presentation

Use of Bacteria in Antibody Production

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Use of Bacteria in Antibody Production Robert Gallo

  2. A big Problem… • There are many diseases in the world caused by pathogens that devastate many people each year, causing pain, financial loss, and death

  3. A Solution… • Use modified E. Coli to create antibodies for certain diseases ahead of time, so that an immune boost can be given via injection, or so a response can begin before it normally would.

  4. Brief background on the Immune System • Your body makes antibodies to fight infections • Antibodies latch onto the receptors on pathogen to mark them for destruction by T-cells • Antibodies also can destroy some pathogens by themselves

  5. How Antibodies are made • Antibodies are made in cells known as B-cells • The genes for antibodies are stored in 3 regions known as the IGH, IGK, and IGL chromosome regions • About 1,000 antibody genes code for 10 Billion Antibodies

  6. Antibody diversity • To make so many antibodies, a process known as V(D)J Recombination occurs • In this, genes are recombined to make the desired antibodies

  7. Engineered E. Coli Solution • First, Human Macrophages are introduced to a pathogen • Upon consumption, the macrophages display a signal on its surface telling the E. coli to begin antibody production • Normally, Helper T-cells receive the signal and send another one to B-cells

  8. What the E. Coli does • Upon receipt of the signal from the macrophage via the T-cell receptor gene spliced in, the E. coli creates a signaling molecule that activates the antibody production genes and the V(D)J recombination gene that were also spliced in. • In addition, GFP will be made along with the antibody to demonstrate that the system is working

  9. Testing • Everything must be tested! • 3 principle tests needed: • First, verify that the macrophages will still eat the pathogen and properly display its signal • Then, verify that the E. Coli interpret the signal correctly and produce antibodies • Last, verify that the antibodies do not cause an immune response

  10. Problems • Probably a lot of bugs that need working out • Might cause an immune response • Probably very expensive in development and cost

  11. Other Options? • Vaccines: They are the best option, as prevention is always better, but they are useless, if not harmful, after infection has begun • Antibiotics: They only work for bacteria, not viruses, but are effective and readily available. They also increase the risk of creating superbugs

  12. Sources • ncbi.nlm.nih.gov/books/NBK26860 • newscientist.com • cellsalive.com/antibody • ebi.ac.uk • lifetechnologies.com • Registry of Standard Biological Parts • http://ghr.nlm.nih.gov/gene/RAG1 • TinkerCell • Wikipedia • https://www.pblassaysci.com/sites/pblassaysci/files/images/Antibody%20structure.jpg • http://www.smcm.edu/gambia/photos/vaccine.jpg • http://4.bp.blogspot.com/-yy1sO-aDry0/TylPVEt1YVI/AAAAAAAAA0g/reR5WHr9Ur4/s1600/i_love_fixing_problems_sticker-p217445233701627500z85xz_400.jpg

More Related