1 / 15

UTACCEL 2010

UTACCEL 2010. Adventures in Biotechnology. Graham Cromar. Drew Endy on Synthetic Biology. Available here: http://ung.igem.org/Videos. Synthetic Biology. A new process for engineering biological systems. An approach to engineering biology. It’s not what you make it’s how you make it.

branxton
Download Presentation

UTACCEL 2010

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. UTACCEL 2010 Adventures in Biotechnology Graham Cromar

  2. Drew Endyon Synthetic Biology Available here: http://ung.igem.org/Videos

  3. Synthetic Biology A new process for engineering biological systems An approach to engineering biology It’s not what you make it’s how you make it. atagtacc 10101010101010 Abstraction Standards Automated construction (made to order dna) Synthetic Biology Genetic Engineering (last 30 years) Automated Sequencing (reading dna) Polymerase Chain reaction (writing dna) Recombinant DNA (restriciton enzymes and ligation)

  4. Recombinant DNA Restriction enzymes • In nature these proteins provide a defense to protect bacteria by cutting foreign DNA into pieces. • This cutting activity is specific to certain short sequences of DNA letters. We call this the “restriction site”. • There are many different restriction enzymes that all cut DNA at different sequences of letters. All are commercially available. Ligation A process which repairs breaks in cut DNA using the enzyme, DNA ligase. Together, these allow us to create new DNA sequences by “cut and paste”

  5. EcoRI

  6. PCR Polymerase Chain Reaction Using a heat cycler and all the molecules and enzymes needed to make DNA we can do this process in a test tube making many many copied of our new piece of DNA. This is called “DNA amplification” A T C C C G A T C C C G A T C C C G T A G G G C T A G G G C T A G G G C T A C G G C A T G C C G A T G C C G T A C G G C A T G C C G T A C G G C A A A a a a

  7. Cloning and Transformation

  8. Progress! Abstraction Standards Automated construction (made to order dna) Synthetic Biology Genetic Engineering (last 30 years) Automated Sequencing (reading dna) Polymerase Chain reaction (writing dna) Recombinant DNA (restriciton enzymes and ligation)

  9. Science vs. Engineering A Scientist discovers that which exists; an Engineer creates that which never was. -- Theodore von Karman Biology is the nanotechnology which works catabolism anabolism Real world complexity Small core of standard parts Design information Abstraction reduces complexity by hiding details. Allows for specialization and better reliability

  10. Standardization Powerful tools of engineering design • abstraction • hierarchy • modularity • standardization • isolation, separation of concerns • flexibility Role of standards in engineering • Simplified thinking about interfaces • Reusable Parts • Contracts and commercial access • Independent evolution of components • Facile comparison of results

  11. Coding Standard Repository of Biological Parts The Registry is a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. Examples of parts Several Standards Promoters Terminators Ribosome binding sites Protein coding regions • Standard components & interfaces • Standard composition • Standard function & interfaces • Standard measurements • Standard chassis Types of devices Protein generators Reporters Inverters Receivers and senders Measurement devices Part:BBa_K192000 A shell-forming protein known as "encapsulin", which we derived from the TM0785 plasmid in T. maritima The parts catalogue can be found at: http://partsregistry.org/Main_Page

  12. iGEM International Genetically Engineered Machine Competition Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. Asia has these 38 teams ArtScienceBangaloreCBNU-KoreaChibaECUST-Shanghai HKU-Hong_KongHKUSTHokkaidoU_JapanHong_Kong-CUHK IBB-PuneIITBombayIIT_Delhi_1IIT_Madras KAIST-KoreaKIT-KyotoKorea_U_SeoulKyoto Macquarie_AustraliaMonash_AustraliaNCTU_FormosaNYMU-Taipei OsakaPekingRMIT_AustraliaSJTU-BioX-Shanghai TianjinTokyo-NoKoGenTokyo_MetropolitanTokyo_Tech TsinghuaTzuChiU_FormosaUQ_AustraliaUSTC USTC_SoftwareUT-TokyoXMU-ChinaZJU-China Fudan-Shanghai SJTU-Oncology-Shanghai http://2010.igem.org/Main_Page

  13. Applications

  14. Applications Engineering bacterial microcompartments to make biochemical reactions more efficient G. Cromar, D. Wong, K. Zhan, Y. Leung, M. Gao, S. Hung, F. Raja and J. Parkinson Fully described at: http://2009.igem.org/Team:TorontoMaRSDiscovery

  15. What would you do? Group Exercise: Poster tour Working in teams of three, use the large paper to create a ‘poster’ describing your ideas of what to make if you could write the genetic code. Can you think of three constructive projects? Three destructive projects? When done tour the posters of the other groups to see their ideas.

More Related