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This study investigates the elution profiles and molecular weights of ClpP and its variants (ClpP1 and ClpP2) using different extracts. The data outlines the observed molecular weights (MW in kDa) from various elution stages, providing insights into the purification process of ClpP proteins. Specific focus is given to the His-tagged versions, ClpP1His and ClpP2His, as well as their behavior in elution. The findings may contribute to understanding the structural properties and functional roles of ClpP in cellular processes.
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Extracts Elution A ClpP ClpP1 ClpP ClpP1 ClpP ClpP1 ClpP ClpP1 MW (kDa) 1 2 3 4 5 6 140.0 100.0 70.0 50.0 40.0 35.0 25.0 15.0 Extracts Elution ClpP1His ClpP2His B ClpP ClpP2 ClpP ClpP2 ClpP ClpP ClpP ClpP2 ClpP ClpP2 MW (kDa) 1 2 3 4 5 6 140.0 100.0 70.0 50.0 40.0 35.0 25.0 15.0 Elution C ClpP ClpP1 ClpP ClpP2 Extract ClpP MW (kDa) ClpP ClpP1 ClpP2 1 2 3 4 5 6 ClpP2His 25.0 ClpP1His ClpP 15.0