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Journal of Industrial Microbiology and Biotechnology Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high level production of L-2,3-butanediol

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Journal of Industrial Microbiology and Biotechnology

Identification and characterization of a short-chain acyl dehydrogenase from Klebsiellapneumoniae and its application for high level production of L-2,3-butanediol

Jang Min Park  Won-Kyung Hong  Sung-Mok Lee  Sun-YeonHeo You Ree Jung  In Yeong Kang Baek-Rock Oh Jeong-Woo Seo () Chul Ho Kim ()

Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea

Tel: +82 63 570 5160; Fax: +82 63 570 5109; E-mail: jwseo@kribb.re.kr (J. W. Seo);

Tel: +82 63 570 5110; Fax: +82 63 570 5109; E-mail: kim3641@kribb.re.kr (C. H. Kim)

Table. S1. Oligomers used to express deydrogenase gene of K. pneumoniae

Restriction enzyme sites: bold capital letter, XbaI; bold italic letters, SpeI; italic letters, XhoI; lined italic letters, BamHI; lined letters, SalI.