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LECTURE 03: PATTERNS OF INHERITANCE II. genetic ratios & rules statistics binomial expansion Poisson distribution sex-linked inheritance cytoplasmic inheritance pedigree analysis. GENETIC RATIOS AND RULES.

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lecture 03 patterns of inheritance ii
LECTURE 03: PATTERNS OF INHERITANCE II
  • genetic ratios & rules
  • statistics
    • binomial expansion
    • Poisson distribution
  • sex-linked inheritance
  • cytoplasmic inheritance
  • pedigree analysis
genetic ratios and rules
GENETIC RATIOS AND RULES
  • product rule: the probability of independent events occurring together is the product of the probabilities of the individual events... AND

A/a x A/a

½ A + ½ a½ A + ½ a

P(a/a) = ½x½ = ¼

  • sum rule: the probability of either of two mutually exclusive events occurring is the sumof the probabilities of the individual events... OR

A/a x A/a

½ A+ ½ a½ A+ ½ a P(A/a) = ¼+¼ = ½

statistics binomial expansion
STATISTICS: BINOMIAL EXPANSION
  • diploid genetic data suited to analysis (2 alleles/gene)
  • examples... coin flipping
  • product and sum rules apply

n

  • use formula: (p+q)n =  [n!/(n-k)!k!] (pn-kqk) = 1

k=0

  • define symbols...
statistics binomial expansion4
STATISTICS: BINOMIAL EXPANSION

n

  • use formula: (p+q)n =  [n!/k!(n-k)!] (pkqn-k) = 1

k=0

  • define symbols...
  • p = probability of 1 outcome, e.g., P(heads)
  • q = probability of the other outcome, e.g., P(tails)
  • n = number of samples, e.g. coin tosses
  • k = number of heads
  • n-k= number of tails
  •  = sum probabilities of combinations in all orders
statistics binomial expansion5
STATISTICS: BINOMIAL EXPANSION

n

  • use formula: (p+q)n =  [n!/k!(n-k)!] (pkqn-k) = 1

k=0

  • e.g., outcomes from monohybrid cross...
  • p = P(A_) = 3/4, q = P(aa) = 1/4
  • k = #A_, n-k = #aa
  • 2 possible outcomes if n = 1: k = 1, n-k = 0 ork = 0, n-k = 1
  • 3 possible outcomes if n = 2: k = 2, n-k = 0 or k = 1, n-k = 1 ork = 0, n-k = 2
  • 4 possible outcomes if n = 3... etc.
statistics binomial expansion6
STATISTICS: BINOMIAL EXPANSION

n

  • use formula: (p+q)n =  [n!/k!(n-k)!] (pkqn-k) = 1

k=0

  • e.g., outcomes from monohybrid cross...
statistics binomial expansion7
STATISTICS: BINOMIAL EXPANSION
  • Q: True breeding black and albino cats have a litter of all black kittens. If these kittens grow up and breed among themselves, what is the probability that at least two of three F2 kittens will be albino?
  • A: First, define symbols and sort out basic genetics... one character – black > albino, one gene B > b ...
statistics binomial expansion9
STATISTICS: BINOMIAL EXPANSION
  • possible outcomes for three kittens...
statistics binomial expansion11
STATISTICS: BINOMIAL EXPANSION

n

  • expansion of (p+q)n =  [n!/k!(n-k)!](pkqn-k) = 1

k=0

(3!/3!0!)(¾)3(¼)0 =  x 27/64 = 27/64 = P(0 albinos)

(3!/2!1!)(¾)2(¼)1 =  x 9/64 = 27/64 = P(1 albino)

(3!/1!2!)(¾)1(¼)2 =  x 3/64 = 9/64 = P(2 albinos)

(3!/0!3!)(¾)0(¼)3 =  x 1/64 = 1/64 = P(3 albinos)

P(at least 2 albinos) = 9/64 + 1/64 = 5/32

  • ... if p(black) = ¾, q(albino) = ¼, n = 3 ...
  • expansion of (p+q)3 = (¾+¼)(¾+¼)(¾+¼ ) =

}

sex linked inheritance13
SEX-LINKED INHERITANCE

 are homogametic

 are heterogametic

sex linked inheritance17
SEX-LINKED INHERITANCE

 all white

 all red

sex linked inheritance18
SEX-LINKED INHERITANCE

the white gene is X-linked

 all red

red and white

slide19

CYTOPLASMIC INHERITANCE

selfing:

  • are there differences between groups?
  • are they true-breeding “genotypes”?
slide20

CYTOPLASMIC INHERITANCE

reciprocal crosses:

  • are differences due to non-autosomal factors?
  • compare  progeny to see cytoplasmic influence
statistics poisson distribution
STATISTICS: POISSON DISTRIBUTION
  • binomial... sample size (n)  10 or 15 at most
  • if n = 103 or even 106 need to use Poisson
  • e.g. if 1 out of 1000 are albinos [P(albino) = 0.001], and 100 individuals are drawn at random, what are the probabilities that there will be 3 albinos ?
  • formula: P(k) = e-np(np)kk!
statistics poisson distribution22
STATISTICS: POISSON DISTRIBUTION
  • formula: P(k) = e-np(np)k k!
  • k (the number of rare events)
  • e= natural log =  (1/1! + 1/2! + … 1/!) = 2.71828…
  • n = 100
  • p = P(albino) = 0.001
  • np =P(albinos in population) = 0.1
  • P(3) = e–0.1(0.1)3/3! = 2.718–0.1(0.001)/6 = 1.5 × 10–4
pedigree analysis
PEDIGREE ANALYSIS
  • pedigree analysis is the starting point for all subsequent studies of genetic conditions in families
  • main method of genetic study in human lineages
  • at least eight types of single-gene inheritance can be analyzed in human pedigrees
  • goals
    • identify mode of inheritance of phenotype
    • identify or predict genotypes and phenotypes of all individuals in the pedigree
    • ... in addition to what ever the question asks
pedigree analysis25
PEDIGREE ANALYSIS
  • order of events for solving pedigrees
    • identify all individuals according number and letter
    • identify individuals according to phenotypes and genotypes where possible
    • for I generation, determine probability of genotypes
    • for I generation, determine probability of passing allele
    • for II generation, determine probability of inheriting allele
    • for II generation, same as 3
    • for II generation, same as 4… etc to finish pedigree
pedigree analysis26
PEDIGREE ANALYSIS
  • additional rules...
    • unaffected individuals mating into a pedigree are assumed to not be carriers
    • always assume the most likely / simple explanation, unless you cannot solve the pedigree, then try the next most likely explanation
pedigree analysis27
PEDIGREE ANALYSIS

Autosomal Recessive: Both sexes affected; unaffected parents can have affected progeny; two affected parents have only affected progeny; trait often skips generations.

pedigree analysis28
PEDIGREE ANALYSIS

Autosomal Dominant: Both sexes affected; two unaffected parents cannot have affected progeny; trait does not skip generations.

pedigree analysis29
PEDIGREE ANALYSIS

X-Linked Recessive: More  affected than ; affected  pass trait to all ; affected  cannot pass trait to ; affected  may be produced by normal carrier  and normal . 

pedigree analysis30
PEDIGREE ANALYSIS

X-Linked Dominant: More  affected than ; affected  pass trait to all  but not to ; unaffected parents cannot have affected progeny. 

pedigree analysis31
PEDIGREE ANALYSIS

Y-Linked: Affected  pass trait to all ;  not affected and not carriers. 

Sex-Limited: Traits found in or in  only. 

Sex-Influenced,  Dominant: More  affected than ; all daughters of affected  are affected; unaffected parents cannot have an affected . 

Sex-Influenced,  Dominant: More  affected than ; all sons of an affected  are affected; unaffected parents cannot have an affected .

pedigree analysis32
PEDIGREE ANALYSIS
  • mode of inheritance ?
    • autosomal recessive 
    • autosomal dominant 
    • X-linked recessive 
    • X-linked dominant 
    • Y-linked 
    • sex limited 
pedigree analysis33
PEDIGREE ANALYSIS
  • if X-linked recessive, what is the probability that III1 will be an affected  ?
pedigree analysis34
PEDIGREE ANALYSIS

A/Y a/a

A/a a/Y

  • genotypes

P(II1 is A/a) = 1

P(II2 is a/Y) = 1

pedigree analysis35
PEDIGREE ANALYSIS

A/Y a/a

A/a a/Y

A a a Y

a/Y

  • genotypes

P(II1 is A/a) = 1

P(II2 is a/Y) = 1

  • gametes

P(II1 passes A) = (1)(½)

P(II1 passes a) = (1)(½)

P(II2 passes a) = (1)(½)

P(II2 passes Y) = (1)(½)

pedigree analysis36
PEDIGREE ANALYSIS

A/Y a/a

A/a a/Y

A a a Y

a/Y

  • genotypes

P(II1 is A/a) = 1

P(II2 is a/Y) = 1

  • gametes

P(II1 passes A) = (1)(½)

P(II1 passes a) = (1)(½)

P(II2 passes a) = (1)(½)

P(II2 passes Y) = (1)(½)

  • P(III1 is affected ) = (1)(½)  (1)(½) = ¼
patterns of inheritance problems
PATTERNS OF INHERITANCE: PROBLEMS
  • in Griffiths chapter 2, beginning on page 62, you should be able to do ALL of the questions
  • begin with the solved problems on page 59 if you are having difficulty
  • check out the CD, especially the pedigree problems
  • try Schaum’s Outline questions in chapter 2, beginning on page 66, and chapter 5, beginning on page 158