Batch-at-a-Glance Powerful & Fast Sample and Compound Navigation Automated Quant Method Editor MRM or SIM, Scan with

Agilent MassHunter Quantitative Analysis • Batch-at-a-Glance • Powerful & Fast Sample and Compound Navigation • Automated Quant Method Editor • MRM or SIM, Scan with Library Search, and 2D signals • Parameter-less Integrator • Auto-magic – no parameters! • Outlier Detection • Over 30 criteria increase productivity • Excel-based Reporting • Powerful Reporting & Customization with Excel + XML integration • Compliance • Flexible features supporting Part 11 High Productivity Software

Agilent MassHunter Quantitative Analysis

Agilent MassHunter Quantitative Analysis • Batch-at-a-Glance • Powerful & Fast Sample and Compound Navigation • Automated Quant Method Editor • MRM or SIM, Scan with Library Search, and 2D signals • Parameter-less Integrator • Auto-magic – no parameters! • Outlier Detection • Over 30+ criteria increase productivity • Excel-based Reporting • Powerful Reporting & Customization with Excel + XML integration • Compliance • Flexible features supporting Part 11

Batch-at-a-Glance provides superior sample & compound navigation Including Compound Group and Time Segment navigation… Sample’s compound information is shown as summary or detailed

Batch-at-a-Glance provides convenient compound navigation Batch-at-a-Glance provides superior compound graphics Batch-at-a-Glance Compound graphics displays qualifier ratios for both target & ISTD

Batch-at-a-Glance Calibration table displays CAL, QC, & ISTD points Batch-at-a-Glance Calibration table shows curve as Concentration or Relative Concentration Batch-at-a-Glance Calibration table highlights curve metrics including disabled points

Sample Information displays entire chromatogram at a glance Sample Information overlays Target and ISTD signals Sample Information displays 2D detector signals {ECD, PID, NPD, …}

Quant Method Editor shows extracted ion chromatograms for target and ISTD Quant Method Editor presents entire method “at-a-Glance” Quant Method Editor enables a logical step by step approach

Quant Method Editorenables stepwise decisions

Quant Method Editor supports: 1. Bracketing2. Relative ISTD 3. Standard Addition

Quant Method Editor automates method creation from Scan data – optionally using Deconvolution then Library Search to ID compounds Quant Method Editor automates setting: 1. Compound Name 2. ISTD Name 3. Extracted ion (transitions) 4. Retention Time 5. Qualifier Ratio Quant Method Editor automates Quant method creation form MRM data

Quant Method Editor update includes shifting Retention Time relative and/or absolute Quant Method Editor provides powerful tools to update your method

Quant Method Editor Advanced Tasks enables 2D signal setup Quant Method Editor enables 2D quantitation for 2D as well as MS signals Quant Method Editor treats 2D signal with a first class citizen

Qualitative Analysis simplified user interface shows only needed features Qualitative Analysis interactive features facilitate method development Qualitative Analysis sister application provides powerful discovery tools

Resp. Factor 1512 RSD 14.0% 100 fg Resp. Factor 1546 RSD 2.0% 1 pg Resp. Factor 1542 RSD 0.9% 10 pg Resp. Factor 1526 RSD 0.8% 100 pg RSD 1.1% Resp. Factor 1009 1 ng Parameter-less Integrator works on high and low QQQ signal levels Parameter-less Integrator does not need any parameters

Parameter-less Integrator classifies integrated peak: Height Area Width Symmetry Merged Spiky Parameter-less Integrator virtually eliminate manual integration below LOD

Outliers highlight batch quality metrics Outliers flag and filter any result outside limits Outliers in black are turned on by default including Integration Metric

Outliers highlight and filter flagged results Outliers improve productivity by zero’ing in on exceptions Outliers save time by highlighting outliers - Accuracy metric is outside limits

Outliers consists of over30+ separate metrics Outliers improve productivity by zero’ing in on exceptions Outliers configured in Method Editor

Excel-based reporting provides over 100 (counts letter and A4) pre-defined templates Excel-based reporting generates electronic or paper documents Excel-based reporting configures graphics

Excel-based reports display high quality configurable graphics Excel-based reports organize one sheet per sample Excel-based reports display summary as well as details in one WorkBook

Excel-based reporting offers a familiar industry standard tool: Excel Excel-based reporting offers automated background printing; even generating Adobe .pdf documents Excel-based reporting offers a flexible output reviewable with the read-only Excel viewer

Excel-based reporting XML documents facilitate integration to LIMs Excel-based reporting reads XML documents Excel-based reporting provides customizable templates

Excel-based reporting leverages from Excel – the analytical tool of choice Excel-based reporting accesses Excel’s Header/Footer customization

Excel-based reporting opens the door to add calculations based on formulas…

Quant features include: 1. Data Security 2. Roles 3. Audit Trail Quant offers software features that support compliance

Agilent MassHunter Quantitative Analysis • Batch-at-a-Glance • Powerful & Fast Sample and Compound Navigation • Automated Quant Method Editor • MRM or SIM, Scan with Library Search, and 2D signals • Parameter-less Integrator • Auto-magic – no parameters! • Outlier Detection • Over 30+ criteria increase productivity • Excel-based Reporting • Powerful Reporting & Customization with Excel + XML integration • Scan features • Deconvolution and Library Search

Library Editor provides filtering and creating sub-libraries Library stores compound’s spectra.

Unknowns Analysis uses deconvolution and library search on a single sample. Unknowns Analysis lists Tentatively Identified Compounds beyond target compounds.

Perceive Compounds enables interactive confirmation of the deconvoluted spectrum Perceive Compounds uses deconvolution and library search

New Method from Scan Data automates creation of Quant method. New Method from Acquired Scan Data with Library Search usesdeconvolution and library search

Batch-at-a-Glance Powerful & Fast Sample and Compound Navigation Automated Quant Method Editor MRM or SIM, Scan with