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AGRICULTURAL UNIVERSITY OF ATHENS

AGRICULTURAL UNIVERSITY OF ATHENS. AGRICULTURAL UNIVERSITY OF ATHENS. Laboratory of Plant Breeding & Biometry. AGRICULTURAL UNIVERSITY OF ATHENS. AGRICULTURAL UNIVERSITY OF ATHENS. Laboratory of Plant Breeding & Biometry. Molecular cytogenetics Species and genomic relationships

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AGRICULTURAL UNIVERSITY OF ATHENS

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  1. AGRICULTURAL UNIVERSITY OF ATHENS AGRICULTURAL UNIVERSITY OF ATHENS Laboratory of Plant Breeding & Biometry

  2. AGRICULTURAL UNIVERSITY OF ATHENS AGRICULTURAL UNIVERSITY OF ATHENS Laboratory of Plant Breeding & Biometry • Molecular cytogenetics • Species and genomic relationships • Genomic changes in interspecific hybrids • Collecting wild species • Repetititive sequences • Retratransposons • In situ conservation • Landraces • Molecular markers

  3. RAPDs RAPDs AFLPs A.abyssynica A.abyssynica AB A.vaviloviana A.vaviloviana A.barbata A.barbata A.brevis A.lusitanica A.strigosa A.brevis As A.matritensis A.strigosa A.lusitanica A.matritensis A.hirtula A.hirtula Ad A.damascena A.damascena Al A.longiglumis A.longiglumis Ap A.prostrata A.prostrata A.sativa A.sativa ACD A.sterilis A.sterilis A.maroccana A.maroccana AC A.murphyi A.murphyi A.clauda Cp A.clauda A.eriantha A.eriantha A.ventricosa A.ventricosa AFLPs Cv r =0,92 Mantel Theoretical and Applied Genetics (2004) Drossou et al.

  4. Evolutionary Network C Ac Al Ap & Ad AC & ACD AC & ACD A.strigosa & A. sativa

  5. 7191 7060 7194 7197 7261 7233 7258 7044 7068 7064 7166 7159 367381 7056 7168 2087 9005 2201 9114 7046 2518 7162 + 6585 19532 * 19438 2521 19487 2080 6317 * 436103 412767 19531 19530 9089 * 2048 A 2190 9071 7429 4719 IMAGE 7054 B 3673 7120 7070 N127 4704 7668 C E D PCOORDA Plot 0.61 0.36 * 0.11 AXIS2 – 9.9% 7433 7041 AXIS2 - 9.9% AXIS2 - 9.9% : As 19437 : Al : Ac - 0.14 : Ad : Ap : AB * : AC : ACD : C : A. elatius + - 0.39 - 0.34 - 0.06 0.22 0.49 0.77 AXIS1 - 18.4%

  6. 5΄ 3΄ ETS ETS 18S ITS-1 5.8S ITS-2 26S 18SE-cl 26SE-cl PCR & Cloning Bulked SCAR-PCR ‘fine’ SCAR-PCR CITS1F CITS1R CITS1F CITS1R Sequencing of the Full length ITS1-5.8S-ITS2 C-genome clones Screening of C-genome Clones in Allopolyploids • PCR & Cloning of the full length ITS1-5.8S-ITS2region ofA. insularis A, A. maroccana 2190, A. sativa cv ‘Image’, A. fatua L. N127, A. sterilis 4704 • Two hundred individual ITS clones of each genotype were screened in bulked (groups of 10) SCARs reactions using the C-genome specific primers (CITS1F and CITS2R) • Groups that gave strong positive signals were further analysed as individuals • One to three putative C-genome clones for each accession were sequenced

  7. AGRICULTURAL UNIVERSITY OF ATHENS AGRICULTURAL UNIVERSITY OF ATHENS Laboratory of Plant Breeding & Biometry Nikoloudakis, N., Katsiotis, A.The origin of the C-genome and cytoplasm of Avena polyploids (2008) Theoretical and Applied Genetics, pp. 1-9. Article in Press. Nikoloudakis, N., Skaracis, G., Katsiotis, A. Evolutionary insights inferred by molecular analysis of the ITS1-5.8S-ITS2 and IGS Avena spp. sequences (2008) Molecular Phylogenetics and Evolution, 46 (1), pp. 102-115. Drossou, A., Katsiotis, A., Leggett, J.M., Loukas, M., Tsakas, S. Genome and species relationships in genus Avena based on RAPD and AFLP molecular markers (2004) Theoretical and Applied Genetics, 109 (1), pp. 48-54. Katsiotis, A., Loukas, M., Heslop-Harrison, J.S. Repetitive DNA, genome and species relationships in Avena and Arrhenatherum (Poaceae) (2000) Annals of Botany, 86 (6), pp. 1135-1142. Katsiotis, A., Hagidimitriou, M., Heslop-Harrison, J.S. The close relationship between the A and B genomes in Avena L. (Poaceae) determined by molecular cytogenetic analysis of total genomic, tandemly and dispersed repetitive DNA sequences (1997) Annals of Botany, 79 (2), pp. 103-109.

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