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H.Ghaderian1 1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran. : Classification. Family : Herpes viridea Sub family : herpes virinea Genus : simplex Specieas:HSV-1 CL 101 ,HSV-2 strain KN 53690

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slide1

H.Ghaderian1

1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran

classification
:Classification
  • Family : Herpes viridea
  • Sub family : herpes virinea
  • Genus : simplex
  • Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690

common name : herpes symplex virus

slide7

Alpha herpes viruses

latently infected

slide9

HSV of the newborn is acquired 3 periods:

in utera

Peripartium (85%)

postpartum

slide10

Incidence of neonatal herpes in 3 form :

1) lesion of the skin , eyes , mouth

slide12

3) Dissiminateddesease in liver

lunges

gland adrenl

gut

neonatal herpes
Neonatal herpes
  • caseof neonatal herpesHSV -1and HSV-2
  • Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause
  • Serologic investigations showed noIgMand IgGantibodies forHSV-1 and HSV-2 in new born
slide14

Immune ResponsesNeonatal

Infected neonates produce HSV-specific IgM antibodies

within 3 weeks of acquisition of the viral infection,

which increaserapidly during the first 2 to 3 months

and may remain for as long as 1 year after neonatal infection develops.

slide15

Lymphocytes from infected infants

alpha-interferon

NeonatesINF –αcompare

with adults with primary HSV infection is decreased.

slide16

Study showed :

The first time infection of the pregnantwomen

may lead to severe illness in pregnancy and

may be virus transmission to newborn.

diagnistic
DIAGNISTIC

1)PCR

The high risk for death requires prompt diagnostic therforesuggestive that of HSV was identified by PCR

Viral DNA was extracted from all specimens

(tracheal aspirate, liver, lungs, and gut)

By using the QIAamp DNA Mini Kit

slide18

primers for the HSV-1: thymidinekinasegene

((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′

(Rev 5′-TTTTCTGCTCCAGGCGGACT-3′)

Primer for the HSV-2: DNA polymerase gene

((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′

(Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′)

The DNA was then used for HSV DNAPCR

slide19

Nucleotide sequence analysis

showed identical sequences in different specimens.

GenBank BLAST tool

highest similarity was

HSV-1 strain CL 101 and HSV-2 strain KN 53690

2 cell culture
2)Cell culture

• Multi nucleatedepithelial cells

treatment
treatment
  • All infants with diagnosed HSV infection

must be treated with an intravenous therapy with acyclovir

(60 mg/kg/day)

  • Antibody Therapy

Antibodies against the surface glycoproteins gB and gDprophylactic and therapeutic agents inHSV infection

slide22

mechanism acyclovir

inactive acyclovir

Viral thimydin kinas

Monophosphteacyclovoir

cellular thimydin kinase

active ))acyclovir three phosphate

is analog to guanin blocked DNApolymerasefunction

prevention
PREVENTION

Cesarean Delivery

cesarean delivery in a woman with active

genital lesions can reduce risk of acquiring HSV to infant

Antiviral Prophylaxis During Pregnancy

the use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety

hsv vaccine
Hsv vaccine

HSV-2 : glycoprotein D subunit

vaccine adjuvanted :monophosphorylipid A(MPL)

efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.