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Figure S1 additional MS/MS Spectra and b, y ion tables of Phosphorylated Peptides in KO and WT samples, whose Debunker or A score is lower than the cut-off value . ( Δ Indicate to phosphorylated residue). SSWWMLNPEGGKSGK S Δ PR. 7. [M+2H] 2+ m/z: 1042.98. MH-H 3 PO 4 2+. y 11.

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slide1

Figure S1 additional MS/MS Spectra and b, y ion tables of Phosphorylated Peptides in KO and WT samples, whose Debunker or A score is lower than the cut-off value. (Δ Indicate to phosphorylated residue)

slide2

SSWWMLNPEGGKSGKSΔPR

7

[M+2H]2+ m/z: 1042.98

MH-H3PO42+

y11

y11-H3PO4

b15

y12-H3PO4

y9-H3PO4

y12

b8

y6-H3PO4

b6

y4-H3PO4

y6

b16-H3PO4

y3

MH-H3PO43+

y13-H2O

y3-H3PO4

y14

b7

b16

b17+H2O

slide3

YSFAPPNTSLNSPSΔPNYSK

10

[M+2H]2+ m/z: 1076.48

y152+

y14

y5

y8

y7

b12-H2O

y15

b12

MH-H3PO42+

y9-H3PO4

y142+

y15-H2O

b4

y7-H3PO4

b14-H3PO4

b11

b3-H2O

b13

b17

y13

b17-H3PO4

y18

y17

slide4

11

TLPHVVNTMPHTSAMNRLTΔPVK

[M+4H]4+ m/z: 631.82

y132+

b153+

b112+

y14-H2O2+

y193+

y3

y42+

b6

b8

y8-H3PO4

b2

y6-H3PO42+

b184+

b113+

y5-H3PO42+

y8

b4

b12-H2O