1) Retrieval of chromosomal locus matched the EST sequence  by Blast search
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1) Retrieval of chromosomal locus matched the EST sequence by Blast search. EST sequence (mRNA). AAAAAAAAA. GATC. M. genomic DNA. 2) Search for the nearest Pst I site from MboI site of EST. P. M. 3) Design of the primers from Pst I site to fit defined conditions. P.

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1) Retrieval of chromosomal locus matched the EST sequence by Blast search

EST sequence

(mRNA)

AAAAAAAAA

GATC

M

genomic DNA

2) Search for the nearest Pst I site from MboI site of EST

P

M

3) Design of the primers from Pst I site to fit defined conditions

P

4) Final selection of primer by Blast Search

P


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