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修飾茶多酚之奈米金應用於前列腺癌治療與成像 PowerPoint Presentation
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Laminin 67R target. Gelatin (EDC 、 NHS integrate Doxorubicin(DOX). Enzyme decomposition (MMP-2 、 MMP-9). Hao -Ying Hsieh ( 謝昊穎 ) * 、 Li-Chu Tsai( 蔡麗珠 ). Target PC-3 cells. FL-Quenched. FL-Activated. 有機高分子研究所 E-mail:zoe77101y@gmail.com. Laminin 67R . EGCG-Au 0. +. nHCl. +.

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Laminin 67R target

Gelatin (EDC、NHS integrate Doxorubicin(DOX)

Enzyme decomposition

(MMP-2 、 MMP-9)

Hao-Ying Hsieh(謝昊穎) *、Li-Chu Tsai(蔡麗珠)

Target PC-3 cells

FL-Quenched

FL-Activated

有機高分子研究所

E-mail:zoe77101y@gmail.com

Laminin 67R

EGCG-Au0

+

nHCl

+

nH2O

+

Ex:480nm

Em:580nm

Doxorubicin(DOX)

M. Frederick Hawthorne,Laminin receptor specific therapeutic gold nanoparticles

(198AuNP-EGCg) show efficacy in treating prostate cancer, PANS,June 25, 2012

Laminin R

EGCG-

 (2008) J.Biol.Chem. 283: 3002-3005

  • Abstract
  • 本篇論文的研究方向是製備具有對前列腺癌(PC-3cells)細胞標的功能且對腫瘤組織內的金屬蛋白酶(Metalloproteinase (MMP-2、MMP-9))具有敏感性的奈米金載體
  • (-)-Epigallocatechingallate (EGCG)-Au Nanoparticles(NPs)以應用於抗癌藥物的標靶傳送與螢光顯影偵測的應用。我們利用EGCG製備出不同吸收波長之奈米金粒子,並將Doxorubicin(DOX)藉由Gelatin結合於EGCG-Au奈米粒子表面,利用EGCG對PC-3細胞的標靶特性及奈米金的NSET效應,再藉由腫瘤組織中的MMP酵素專一性分解GLT,此奈米粒子具有應用於前列腺癌之標靶、專一性偵測與治療之潛力。

Introduction

根據2010年美國防癌協會(American Cancer Society)資料指出,癌症共有一百多種,其中前列腺癌在美國男性中是第二大致命症,每年有超過二十萬新病例。尤其是50嵗以上的男性,前列腺癌的患病機率亦隨之增高,所以美國防癌協會建議50嵗以上的男士,每年做肛門指探診(Digial rectal examination,DRE)以及前列腺抗體驗血檢查(Prostate specific antigen,PSA),通過檢查可早期發現前列腺癌並及時治療1。

修飾茶多酚之奈米金應用於前列腺癌治療與成像修飾茶多酚之奈米金應用於前列腺癌治療與成像

Surgical resection

Prostate cancer’streatment

EGCG

Androgen

Results and Discussion

1.傅立葉紅外光譜結果(FT-IR)

2.界達表面電位結果(Zeta potential)

Radiotherapy

Source: Human health wikipedia

Chemotherapy

4.穿透式電子顯微鏡-元素分析結果

3.螢光光譜猝火結果(FL-Quenched)

  • 6.前列腺癌細胞(PC-3)標靶顯影結果

5.細胞抑制率測試(MTTassay)

Experiments

Conclusion

本實驗成功的利用EGCG製備出不同吸收波長之奈米金粒子,並將DOX藉由GLT結合於EGCG-Au奈米粒子表面,利用EGCG對PC-3細胞的標靶特性及奈米金的NSET效應,再藉由腫瘤組織中的MMP酵素專一性分解GLT,此奈米粒子具有應用於前列腺癌之標靶、專一性偵測與治療之潛力。

  • References
  • 1.AmericanCancerSociety(2010)CancerFactsandFigures2010(AmericanCancerSociety,Atlanta),pp23–35.
  • 2.H.S.Yoo,K.H.Lee,J.E.Oh,T.G.Park,JournalofControlled Release68(2000)419
  • 3.S.Mitra,U.Gaur,P.C.Ghosh,A.N.Maitra,JournalofControlled Release74(2001)317.
  • 4.S.E.Gelperina,A.S.Khalansky,I.N.Skidan,Z.S.Smirnovac,A.I.Bobruskin,S.E.Severin,B.Turowski,F.E.Zanella,J.Kreuter,ToxicologyLetters126(2002)131