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Impact of Gasoline on Aiptasia Symbiosis with Zooxanthellae in Controlled Tank Study

Investigating the effects of gasoline on coral reef health by analyzing the symbiotic relationship between Aiptasia and Zooxanthellae in a tank simulation. The study aims to determine if gasoline disrupts this symbiosis. Key procedures include adding gasoline to the tank and analyzing zooxanthellae density in Aiptasia. Results will be analyzed for statistical significance. Considerations include Hawaiian species, unwanted Aiptasia presence, and proper statistical analysis.

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Impact of Gasoline on Aiptasia Symbiosis with Zooxanthellae in Controlled Tank Study

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  1. The effects of Gasoline on Aiptasia Group 4: Joon Chung Jimmy Wesman Heather Hollembeak Sebastian Camacho Nauman Chandry Will Quartell

  2. Background Information • Problem: Many boater’s leak fuel into the ocean which may harm coral reefs. • We want to simulate this occurrence in our tank to see if the symbiosis between aiptasia and zooxanthellae is disrupted

  3. Hypotheses • H0: The addition of gasoline will not affect zooxanthellae density in aiptasia. • HA: The addition of gasoline will reduce zooxanthellae density in aiptasia.

  4. Clarification Bleaching is a descriptive term

  5. Procedure • November 1- We added .64 ml of 87 octane gasoline to the tank and turned the filter off. (according to CEAA) • November 8- Originally supposed to add .64 ml more • November 15- Decide whether to add more gasoline and if so how much? (Take into account Thanksgiving Holiday)

  6. Procedure Continued • Cell count using hemocytometer • Chlorophyll extraction and measurement using acetone • Protein concentration using Bradford method

  7. Results

  8. Things to think about • Statistical Significance • Hawaiian Species • Unwanted Aiptasia • A mollusk walks up to a sea cucumber and http://www.youtube.com/watch?v=CSU83KfjcWE

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