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This study focuses on genetic manipulation of colonies to express GFP using Ribo constructs. By utilizing the 3’UTR and HDV sequences, we engineered the colonies to produce bright green fluorescence. The constructs pBG60 and pBG78 were key in achieving this modification. This research delves into the molecular mechanisms behind GFP expression in these genetically modified colonies.
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A B 1 Kb BG192 SGP2 bsd attB1 GFP attB2 3’UTR HDV Ribo BG209 pBG60 pBG78 colonies 1 Kb Phase GFP C