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Supplementary Fig. 1. Gated on lymphocytes. TCR- gd. CD161. SSC. Total PBMC. Gated on ab T cells. CD3. FSC. 3C10 (V a 7.2). Supplementary Fig. 1: Gating strategy for MAIT cells.

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Fsc

Supplementary Fig. 1

Gated on lymphocytes

TCR-gd

CD161

SSC

Total PBMC

Gated on abT cells

CD3

FSC

3C10 (Va7.2)

Supplementary Fig. 1: Gating strategy for MAIT cells.

PBMC were stained with mAbs against CD3, TCR-gd, CD161, and 3C10 (Va7.2), and gated on lymphocyte (left), CD3+TCR-gd- (middle), and then on 3C10+CD161hi (right) cells to identify MAIT cells as reported by Martin et al (11).


Fsc

Supplementary Fig. 2

*

*

Supplementary Fig. 2: IFN-g production from PBMC depleted with MAIT cells in response to CD3 or CD3 and CD28 stimulation.

PBMC with (DMAIT) or without (Total) depletion of MAIT cells were stimulated with plate-bound anti-CD3 mAb (5 mg/ml) alone (left) or in combination with soluble anti-CD28 mAb (1mg/ml) (right). IFN-g in the supernatant was measured at 48 hours. Results from 4 HC are shown. *p<0.05 (Paired t-test)


Fsc

Supplementary Fig. 3

HC

MS

IL-17

IL- 4

IL- 10

Fig. S3: IL-17, IL-4 and IL-10 production from PBMC depleted with MAIT cells in response to PHA.

IL-17, IL-4, and IL-10 production from PBMC stimulated with PHA (2mg/ml) with (DMAIT) or without (Total) depletion of MAIT cells in HC and MS-rem (N = 6 each).


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