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Learn about specimen collection and identification techniques, decision-making tools, and risk assessment in the context of invasive species. Discover various online resources and databases for ID. Apply DNA barcoding principles. Establish a port ID reference collection.
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- Unit VI -IAS Specimen Collection, Identification, and Preservation Rebecca M. Westbrooks Randy G. WestbrooksSteven Manning Global Invasive Species ProgrammeCape Town, South Africa
GISP Prevention Training Course.Day Five – 15 December, 2006. • 0900-0915. Field Trip Evaluation.(Sandy Johnson). • 0915-0945. Unit 6 - IAS Specimen Collection and Identification.(Becky Westbrooks). • 0945-1015. Unit 7 – IAS Decisionmaking Tools, IAS Risk Assessment(Randy Westbrooks). • 1015-1030. Tea. • 1030-1100. Unit 7 Lab Exercise - Weed Risk Assessment – Beach Vitex. • 1100-1130. Basic Elements of a National IAS Prevention Strategy. (Steve Manning). • 1130-1200.The Way Forward - Workshop Summary and Next Steps for IAS Collaboration and Partnering in Eastern, Central, and Southern Africa. (Lynn Jackson, Becky Westbrooks, Steve Manning, Randy Westbrooks). • 1200-1230.Goodbyes, Pictures, Contact Directory.
Specimen Collection and ID • 6.1. Specimen Collection Guidelines • Wild Populations – Minimal # of Specimens • Port Collections – Adequate # Specimens for Identification • 6.2. Specimen ID Resources • Numerous Online Resources (Textbook P. 170)
6.3. Traditional Dichotomous Keys • Series of Successive Couplets • Couplet – Describes some Feature of Organism being Identified • Couplet 1. Seeds Round Soybean Seeds Oblong Go to #2 • Couplet 2. Seeds White Navy Beans Seeds Black Cuban Black Beans
6.4. Computer Assisted ID – Online Resources • Arbor Day Foundation • Tree ID Key • Amateur Entomology Key to Insect ID • Earth Life Web Production • Online Key to Insect Orders • Lucid Online ID Keys • Numerous Online Keys for Insects, Plants, and etc. • Lucid Key for U.S. Federal Noxious Weed Seeds (USDA APHIS) • Lucid Key for Scale Insects (USDA APHIS)
6.5. Online Invasive Species Databases. • Global Invasive Species Network • Coordinated by USGS BRD • Global Invasive Species Database • World Conservation Unit – Invasive Species Specialist Group – Based in New Zealand • Plants Database • USDA Natural Resources Conservation Service
6.6. DNA Bar Coding – Consortium for the Bar Code of Life (CBOL) • Practical Methods for Rapid ID of the estimated 10 Million Species of Life of Earth • Global Standard in Taxonomy – Especially for Non-Taxonomists • 130 Member Organizations in 40+ Countries (Botanical Gardens, Zoos, Herbaria, etc.) Louisiana Crayfish(Procambarus clarkii)
Principles of DNA Bar Coding • Short Gene Sequence from Standardized Region of the Genome • DNA Bar Coding – Outgrowth of Molecular Biology
6.7. Port ID Reference Collection • Specimen Collection • Wild Populations • Specimens Collected in the Port • Specimen Preparation • Insects • Plants • Seeds
Citrus Blackspot Disease General Beauregard Newton 6.7. Port ID Reference Collection • Plant Diseases • Photographs • Aquatic Nuisance Species • Preserved Specimens • Photographs • Dried Specimens • Injurious Wildlife • Mounted Specimens
Unknown Specimens Collected on Beach in Dar es Salaam, TNZ ?? ?? ?? Recommendation: Put in Quarantine and/or Fumigate until Positively Identified