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WB: a pTyr a ShcA a pTyr a EGFR

Suppl.G. Vec ShcC2 3YF 1 3YF 2. Vec ShcC2 3YF1 3YF2. EGF 100ng/ml 10min. a ShcC. - + - + - + - +. 250. WB: a pTyr a ShcA a pTyr a EGFR. 150. 100. a -tubulin. 75. 50. IP: a ShcA. ShcC2 3YF1 3YF2.

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WB: a pTyr a ShcA a pTyr a EGFR

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  1. Suppl.G Vec ShcC2 3YF 1 3YF 2 Vec ShcC2 3YF1 3YF2 EGF 100ng/ml 10min aShcC - + - + - + - + 250 WB: a pTyr aShcA a pTyr aEGFR 150 100 a-tubulin 75 50 IP: aShcA ShcC2 3YF1 3YF2 - + - + - + WB: a pTyr aShcC IP: aEGFR IP: aShcC Negative regulation of ShcA-phosphorylation by ShcC does not require tyrosine phosphorylation sites of ShcC. The effect of 3YF mutant of ShcC which lacks tyrosine phosphorylation sites on the phosphorylation of ShcA was analyzed by the same methods described in Figure 5 using two stable clones of KU-YS cells expressing 3YF mutants. The 3YF mutant suppressed phosphorylation of ShcA induced by stimulation of EGF to similar levels of p52ShcC (ShcC2). 3YF : a mutant of ShcC in which Y221/222 and Y304 are changed to three phenylalanines (3YF) causing inhibition of signals downstream of Grb2.

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