kinesin sara van nortwick geometric computations in molecular biology ss 2007 eth l.
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Kinesin Sara Van Nortwick Geometric Computations in Molecular Biology SS 2007 ETH. The Structure. X-ray crystallography by Kull et al. (1996) Resolution = 1.8 A Dimer. Let’s Motor!. The Family of Motor Proteins Large and varied responsibilities Unidirectional movement Fuel = ATP

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Presentation Transcript
the structure
The Structure
  • X-ray crystallography by Kull et al. (1996)
      • Resolution = 1.8 A
  • Dimer
let s motor
Let’s Motor!
  • The Family of Motor Proteins
      • Large and varied responsibilities
      • Unidirectional movement
      • Fuel = ATP
      • Share motor “head” domain (structural core, common ancestor)
where are we going and why
Where are we Going and Why?
  • Center Periphery
  • Compass = Microtubule Polarity
    • Anterograde Transport (towards the positive)
  • Point A to Point B
    • Kinesin > Diffusion
  • Alignment
    • Mitosis (cell division)
how fast are we talking
How Fast are we Talking?
  • 2 um/sec
  • 8 nm per step (spacing of tubulin dimers along the microtubule)
  • = 250 steps/second!
  • Protein head = 7.5 X 4.5 X 4.5 nm
  • ≈ speed and thrust of the supersonic car
  • 60% efficiency
the motor in action
The Motor in Action
  • Kinesin Movement Video:
    • http://www.scripps.edu/cb/milligan/research/movies/kinesin_mov.html
many varieties
Many Varieties…
  • Motor domain conserved @ N-terminus
  • 40 known variations in Humans
  • Differences in adaptor structure between motor

and the object to be moved

breaking it down
Two heavy chains comprise globular head

Light Chains comprise cargo binding region

Breaking it Down
kinesin vs myosin
Motor domain is similar in both motor proteins

Very little amino acid similarity

Kinesin is 340 aa while myosin is 850 aa

RMS of 3.5 A for 183 aa of kinesin motor domain

Kinesin Vs. Myosin
references
References
  • Kozeilski, Sack, et al. The Crystal Structure of Dimeric Kinesin and Implication fo Microtubule-Dependent Motility. Cell, Vol 91, 985-994. December, 26, 1997.
  • Molecular Biology of the Cell http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.figgrp.3054
  • PDB Molecule of the Month
  • Kull, Sablin, et al.(1996). Crystal structure of the kinesin motor domains reveals a structural similarity to myosin. Nature 380, 550-554.
  • Wikipedia
  • Block, Steve. Kinesin: What Gives? Cell, Vol 93 5-8. April, 1998.