in silico feedback for in vivo regulation of a gene expression circuit
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In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit. Andreas Milias-Argeitis , Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta , David Pincus , Hana El- Samad , Mustafa Khammash , and John Lygeros Nature Biotechnology November 6 th , 2011 Jamal Elkhader

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in silico feedback for in vivo regulation of a gene expression circuit

In SilicoFeedback for In Vivo Regulation of a Gene Expression Circuit.

Andreas Milias-Argeitis, Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta, David Pincus, Hana El-Samad, Mustafa Khammash, and John Lygeros

Nature Biotechnology

November 6th, 2011

Jamal Elkhader

20.385 Journal Club II

feedback control
Feedback Control
  • Regulation of Cell Behavior
  • Feedback Control Important for Regulation
    • Processing measurements in real time
    • Determining inputs
  • Robust regulation can be achieved!
in silico feedback control
In SilicoFeedback Control
  • In silico– Performed on Computer or via Computer Simulation
  • In silicofeedback
    • Computer
    • Real time data
    • Inputs
    • Regulation
implementation
Implementation
  • S. cerevisiae
  • Light-switchable system (PhyB-PIF3)
  • Transformed cells
    • Grown in Dark
    • PCB
exposure to light
Exposure to Light
  • Red (650 nm) and Far Red (730 nm) Pulses
  • Input – Light Pulses
  • YFP expression
  • Output - YFP
using a kalman filter
Using a Kalman Filter
  • Real Time Regulation
significance
Significance
  • Enhancement of expression
  • Electronic control over living cells
  • Therapeutics
    • Regulation of expression
    • Regulation of toxic by-products
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