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Molecular epidemiology of BVD during the final phase of the Swedish BVD-programme. Photo courtesy http:// philip.greenspun.com. Background Swedish BVD-programme. Running since 1993 Based on increased herd biosecurity in free herds & identification and elimination of carrier animals

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molecular epidemiology of bvd during the final phase of the swedish bvd programme
Molecular epidemiology of BVD during the final phase of the Swedish BVD-programme

Photo courtesy http:// philip.greenspun.com

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

background swedish bvd programme
Background Swedish BVD-programme
  • Running since 1993
  • Based on increased herd biosecurity in free herds & identification and elimination of carrier animals
  • All dairy and beef herds affiliated
  • 96.3 % of herds officially free

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

background swedish bvd programme3
Background Swedish BVD-programme
  • 1996 1997 1998 1999 2000 2001 2002 2003 2004

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

background swedish bvd programme4
Background Swedish BVD-programme
  • New infections are occasionally being detected
  • Established routines to trace sources of new infections

However,in 40-50 % of cases where new infections are

detected in previously free herds, the source remains unknown

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide5

Molecular epidemiology of BVDbackground

BVDV

type-2

The situation in Sweden

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide6

Molecular epidemiology of BVDbackground

BVDV

type-2

  • Subtypes 1a, 1b & 1d

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide7

Molecular epidemiology of BVDbackground

BVDV

type-2

  • Subtypes 1a, 1b & 1d
  • Herd specific clustering
  • Unique isolates

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide8
To build up a bank of BVDV isolates from all infected herds

To characterise BVDV strains circulating in Sweden

Molecular epidemiology of BVDaims

  • Unique possibility to trace routes of infection
  • Surveillance of the national BVDV situation
  • Speed up the final phase of the programme

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide9
Sera from all viruspositive individuals detected between Oct -02 and June -04

One isolate from each infected herd selected

5´NCR used for phylogenetic analysis

Results from phylogenetic analysis compared with reports on suspected transmission routes

Molecular epidemiology of BVDMaterial & methods

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide10

Molecular epidemiology of BVDResults & discussion

  • 329 individuals from 112 herds tested viruspositive
  • 112 isolates selected for further analysis

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide11

Molecular epidemiology of BVDResults & discussion

  • 329 individuals from 112 herds tested viruspositive
  • 112 isolates selected for further analysis
  • 7 isolates of subtype 1a

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide12

Molecular epidemiology of BVDResults & discussion

  • 329 individuals from 112 herds tested viruspositive
  • 112 isolates selected for further analysis
  • 7 isolates of subtype 1a
  • 28 isolates of subtype 1b

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide13

Molecular epidemiology of BVDResults & discussion

  • 329 individuals from 112 herds tested viruspositive
  • 112 isolates selected for further analysis
  • 7 isolates of subtype 1a
  • 28 isolates of subtype 1b
  • 77 isolates of subtype 1d

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide14

67 different sequences distinguished

  • 19 groups of identical sequences

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide15

67 different sequences distinguished

  • 19 groups of identical sequences

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide16

We found cases, where phylogenetic analysis supported suspected transmission routes:

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide17

We found cases, where phylogenetic analysis supported suspected transmission routes:

Case A

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide18

We found cases, where phylogenetic analysis supported suspected transmission routes:

Case B

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide19

We found cases, where phylogenetic analysis supported suspected transmission routes:

Case C

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide20
BVDV type1, subtypes 1a, 1b, 1d

Sequences not unique

Confirm or rule out suspected transmission routes

Valuable tool in any attempt to control BVDV

Molecular epidemiology of BVDconclusions

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide21
Further sequencing of Npro and E2 region

Herd specific clustering

Regional variation- spatial analysis

Molecular epidemiology of BVD coming projects

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide22
Ann Lindberg, DVM, PhD, Swedish Dairy Association, Uppsala, Sweden

Claudia Baule, PhD, National Veterinary Institute, Uppsala, Sweden

Mats Isaksson, National Veterinary Institute, Uppsala, Sweden

Jaruwan Kampa, SLU, Uppsala, Sweden

Stefan Alenius, DVM, PhD, SLU, Uppsala, Sweden

This study was supported by the Swedish Farmers’ Foundation for Agricultural Research (SLF; Project no. 0330007)

Molecular epidemiology of BVDacknowledgements

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden

slide23

Molecular epidemiology of BVD

Karl Ståhl

Dep. of Biomedical Sciences and Veterinary Public health

SLU, Uppsala, Sweden