1 / 28

early shoot borer

early shoot borer

awais16
Download Presentation

early shoot borer

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Muhammad Awais Ismail Regd. No: BSAGL-13-11 Supervisor Dr. SaqiKosarAbbas Parasitic effect of larval ectoparasitoid Braconhebetoron sugarcane early shoot borer Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  2. Sugarcane is the second largest cash crop of Pakistan • It is cultivated on 0.966 million hectares • It contributing around 3.6 % of Gross domestic production (GDP). • Sugarcane currently accounts 4.8% of cropped area • 11% value added of the total Crops. ( M. Asghar Qureshi and Shahid Afghan;2017) INTRODUCTION Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  3. Sugarcane top borer Sugarcane early shoot borer Sugarcane gurdaspur borer Sugarcane aphid Sugarcane mealy bug Sugar cane grub Sugarcane white grub Sugarcane pyrilla Sugarcane pest

  4. Scientific classification of sugarcane early shoot borer • Phylum – Arthropoda • Class – Insecta • Order – Lepidoptera • Family – Pyralidae • Genus – Chilo • Species – infuscatellus • Scintific name - Chiloinfuscatellus Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  5. adult 7-9 days pupa eggs Life cycle 7 days 21 days larvae Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  6. Infestation of pest in internodes makes the matured cane hard • Tunnels are also formed within the shoot • Quality and quantity of the juice is reduced • Damage done by this pest may be up to 50%. Damage Symptoms Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  7. It is a major pest of sugarcane and is prevalent in all the cane growing areas of Pakistan. Nature of Damage: DISTRIBUTION. Fig : stem damage Fig: stem damage Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  8. For many years, the management of lepidopteran are control by different pesticides. • Moreover, insecticides pose a direct risk to human health and the environment due to the presence of their residue in food products and in processing facilities where workers are exposed (Fields and White, 2010) • In recent years, interests have been focused for development of non-chemical strategies( Phillips,2006) • Of these strategies, the use of natural enemies, including parasitoids and predators is an important component of IPM and has many advantages over chemical control (Scholleret al. 1997; Scholler and Flinn, 2000) . Control practices Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  9. Kingdom Animalia • Phylum Arthropoda • Class Insecta • Order Hymenoptera • Family Braconidae • Genus Habrobracon • Species B. hebetor Scientific classification of Braconhebetor

  10. It attacks the larval stages of several species of Lepidoptera including all types of larvae (Mohapatraet al., 2008) • Braconhebetor is a highly polyphagous gregarious ecto-parasitoid of several species of lepidopteran larvae.(Mohapatra et al., 2008) Conti… Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  11. Braconhebetor (Hymenoptera: Braconidae) is an idiobiont, gregarious ectoparasitoid that attacks a variety of important Lepidoptera's pests of stored product. The biology of B. hebetor has been intensively studied because of its importance as a biological control agent of the moths and also it is easy to rear in the laboratory. (Gündüz et al., 2008). Conti….. Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  12. ADULT 13 days Adult egg laying PUPA 2 days Life cycle LARVAE Eggs 1day Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  13. To check the maximum parasitation rate among different host stages • Among three host stages which host is batter for female reproductive fitness Objectives

  14. IDENTIFICATION Female Male Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  15. For many years, the management of lepidopteran pests has traditionally involved the use of fumigants, aerosols and other chemical insecticides. However, these moth species have become resistant to insecticides (Zettleret al.,1973). • Moreover, insecticides pose a direct risk to human health and the environment due to the presence of their residue in food products and in processing facilities where workers are exposed (Fields and White, 2002). • For many years, the management of lepidopteran pests has traditionally involved the use of fumigants, aerosols and other chemical insecticides. However, these moth species have become resistant to insecticides (Zettleret al.,1973). • Moreover, insecticides pose a direct risk to human health and the environment due to the presence of their residue in food products and in processing facilities where workers are exposed (Fields and White, 2002).

  16. Location of Experiment The experiment were carried out in IPM lab, BZU, Bahadur Sub Campus Layyah • Insect Collection Insect parasitoid Braconhebetorwere taken from Insect Molecular Biology Lab, University of agriculture Faislabad. While host insect sugarcane shoot borer was collected from the surroundings of layyah • Rearing of Insects Both the parasitoid and host culture was reared on in the incubator at Temperature of 28±2 ⁰C and 60±5% R.H in the IPM laboratory of BZU Bahadur sub-Campus Layyah Materials and Methods

  17. The adults of the parasitoid was reared in the laboratory, on the larvae of sugarcane shoot borer as a host by using glass vials of (2 cm × 10 cm) • Each vial contains 3rd , 4th and 5thinstar larvae of the host and one fertilized female of the parasitoid,provided with cotton swabs/ pads soaked in 50% honey and water as food source for B. hebetoradult. • A parasitization time of 24 hour was provided to each host instar larvae • After 24 hours, the parasitoid female was take off from each glass vials • The data regarding the parasitoid egg laying, larvae, pupae and adult emergence was recorded on daily basis Bioassay

  18. 3rd instar • 4th instar • 5th instar Total replication: Treatment* Replication (3*5): 15 Parameters • Number of Eggs laying/host larvae • Number of Larvae • Number of pupae formation • Number of adult emergence • Male and female sex ratio Treatment

  19. The data will be statistically analyzed by using 8.1(analytical software) • Treatments means will be separated by Tukey HSD test at 5% probability level(p Statistical Analysis

  20. Data recording

  21. Results

  22. For the parasitic effects of Braconhebetor on the different stages of host larvae, sugarcane shoot borer Analysis of variance (ANOVA)(Table.1) Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  23. Table 1: ) cont.…. Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  24. Mean number of eggs, larvae, pupae and percentage of male and female emergence by the parasitization different stages of sugarcane shoot borer Department of Entomology College of Agriculture BZU, Bahadur Sub Campus Layyah

  25. Akinkurolere R.O., Boyer S., Chen H. & Zhang H. 2009: Parasitism • and host-location preference in Habrobraconhebetor • (Hymenoptera: Braconidae): Role of refuge, choice, and host • instar. — J. Econ. Entomol. 102: 610–615. • Baker J.E. & Fabrick J.A. 2000: Host hemolymph proteins and • protein digestion in larval Habrobraconhebetor (Hymenoptera: • Braconidae). — Insect Biochem. Mol. Biol. 30: 937–946. • Bandani A.R., Kazzazi M. & Mehrabadi M. 2009: Purification • and characterization of midgut α-amylases of Eurygasterintegriceps. • — Entomol. Sci. 12: 25–32. References

More Related