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Referred samples. Outbreak/ Surveillance Sample. Isolates /clinical samples. Record History on different forms`. Sample Collection. Aliquot & Storage. NPA Sample. NS & TS Samples. Sample considered - ve. Isolation. MDCK. Direct and Indirect Immunoflurescence Test. Flu + ve. EGG.

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Presentation Transcript
Flu ve

Referred samples

Outbreak/ Surveillance Sample

Isolates /clinical samples

Record History on different forms`

Sample Collection

Aliquot & Storage

NPA Sample

NS & TS Samples

Sample considered

-ve

Isolation

MDCK

Direct and Indirect Immunoflurescence Test

Flu +ve

EGG

No Isolation

2nd Passage

No Isolation

Low titer Sample >1:8

Low titer Sample >1:8

Haemagglutination Test

Test Results

Verify Sample Titer

Verify Sample Titer

Diagnostic PCR for Low Titer

Genetic Analysis

HA +ve

Haemagglutination Inhibition test


Flu ve

Immune Sera Preparation

Genetic Analysis

Virus Preparation

PCR

Pre Serum Collection

Immunization of Fowl

Sequencing

Post Serum Collecton

Sequence Analysis

Post Booster Serum Collection

Collect Immune Serum


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