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Consortium for Functional Glycomics Pamela A. Marino, Program Director

Consortium for Functional Glycomics Pamela A. Marino, Program Director. Consortium for Functional Glycomics. http://www.functionalglycomics.org. Define paradigms by which protein-carbohydrate interactions mediate cell communication. NIGMS sponsored 'glue grant' (~$8 MM/yr) most $ in cores.

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Consortium for Functional Glycomics Pamela A. Marino, Program Director

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  1. Consortium for Functional GlycomicsPamela A. Marino, Program Director

  2. Consortium for Functional Glycomics http://www.functionalglycomics.org Define paradigms by which protein-carbohydrate interactions mediate cell communication • NIGMS sponsored 'glue grant' (~$8 MM/yr) most $ in cores. • Over 250 participating investigators worldwide • Focus in innate and acquired immunity

  3. Consortium for Functional Glycomics Mouse Phenotype UC San Diego Mouse Transgenics Create & Distribute KO mice TSRI Glycan Library Tools for specificity analysis and biology TSRI • Part. Investigators • Explore Biology using tools • Contribute data • > 80 Institutions Glycan Specificity GBP ligand specificity U Oklahoma Analytical Glycan Profiling Imperial College Gene Microarray GBP and GT genes TSRI Steering Committee Consortium Databases MIT

  4. Structure of the Alliance NCI Tumor Glycome Laboratories NIGMS – CFG Cores Databases Analytical Chemistry Synthetic Chemistry GlycoT Gene Arrays Glycan Arrays NCI – EDRN Components DMCC Coordination Unit

  5. Glycan array screening Glyco-gene microarry Consortium for Functional Glycomics : Glycobiology Resources Offered Glycan specificity of your carbohydrate binding protein on an array with 300 unique glycans representing glycoprotein and glycolipid glycans Analysis of your RNA samples on a highly annotated custom gene microarray with 1800 human and mouse genes Mouse knockout strains Carbohydrate compounds 30 mouse strains with genes ablated for glycosyltransferase and glycan-binding proteins Over 100 carbohydrate compounds represented on the glycan array are available with azido or biotin functional groups http://www.functionalglycomics.org

  6. Analytical Glycomics Core • Human Glycomics: MALDI profiling of N- and O-glycans of tissues, primary cells. • Murine (and human) glycan profile data disseminated on website.

  7. Glycan Array Core • Printed Glycan array expanded to 300 glycans. • Perform Analysis of glycan binding proteins. • Results updated regularly on website. . SNA

  8. Focused Glycogene Microarray • GLYCOv3 chip with up to date gene list highly annotated by participating investigators • Genes for: Glycosyltransferases; Glycan-binding proteins,Glycan degradation proteins; Notch pathway proteins; Nucleotide sugar synthesis and transporter proteins; N-glycan biosynthesis-related proteins; Adhesion molecules; Interluekins and receptors; Mucins; Growth factors and receptors; Cytokines; Chemokines; Conserved oligomeric Golgi (COG) complex proteins • Data entered into database and posted on website.

  9. Mouse Transgenics / Phenotyping Cores • Over 30 KO lines available • Phenotypic data on website

  10. CFG DATABASES • Glycan Binding Protein Database: Molecule pages for 150 glycan-binding proteins • Glycan Structural Database: Over 7500 entries for N-linked, O-linked and synthetic glycans contain structural and chemical information, as well as PubMed links to the source articles. • Glycosyltransferase Database: Glycosylation pathways pages for different glycoconjugate classes. Arrows between structures link to the appropriate CAZY database page for the GT. • power point demo of data bases here: http://www.functionalglycomics.org/static/consortium/CFG_Bioinformatics_Progress_2006.ppt

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