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DNA Fingerprinting. DNA Fingerprinting. In DNA Fingerprinting, the DNA of an organism is cut up into fragments using restriction enzymes producing a large number of short fragments of DNA Because no two individuals have identical DNA, no two individuals will have the same length fragments.

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dna fingerprinting1
DNA Fingerprinting
  • In DNA Fingerprinting, the DNA of an organism is cut up into fragments using restriction enzymes producing a large number of short fragments of DNA
  • Because no two individuals have identical DNA, no two individuals will have the same length fragments
gel electrophoresis
Gel Electrophoresis
  • Gel electrophoresis is a technique used to separate the DNA fragments according to their size
  • The fragments are placed in wells on a sheet of gelatin, and an electric current is applied to the sheet
slide5
The smallest fragments will move the fastest because they are able to move through the pores in the gelatin faster
  • Bands will be produced on the gelatin where the fragments accumulate
slide8
Used to analyzegenes to test for presence of different alleles
    • If a person was homozygous for a trait, the DNA would be cut by the same restriction enzyme in the same place because alleles are the same

Allele from Dad

Allele from Mom

slide9
If a person was heterozygous for a trait, the DNA would be cut by the same restriction enzyme in different places because the alleles are different

Allele from Dad

Allele from Mom

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