Determination of glycan structure use ms ms
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Determination of Glycan structure use MS/MS . Problem Structure of glycan is diverse- complex tree structure, isomeric molecules, number of glycan. Glycan is secondary gene products, hard to predict from genome sequence. Double counting peaks. Current method

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Determination of glycan structure use ms ms
Determination of Glycan structure use MS/MS.

  • Problem

    • Structure of glycan is diverse- complex tree structure, isomeric molecules, number of glycan.

    • Glycan is secondary gene products, hard to predict from genome sequence.

    • Double counting peaks.

  • Current method

    • De novo sequencing from tandem MS data.

    • Generate candidate glycan structure then score with experimental spectrum.

    • 24 spectra identified 58% glycan topologies, each topologies in Top 4 ranking.

  • Our method

    • Using biology prior knowledge to limit the number of candidate glycan. Reduce sample space.

    • Incorporate with HCD data to determinate the linkage.