1 / 3

Study Design

GBV-C blunts T cell activation & proliferation GBV-C induces chemokines GBV-C CCR5 and CXCR4 on CD4 cells GBV-C replicates in T and B cells. P=0.01. N=11. N=13. N=11. N=9. Study Design. ns. UIHC HIV Clinic Patients Invitation – Informed Consent Heparinized Blood/Serum Obtained

artie
Download Presentation

Study Design

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. GBV-C blunts T cell activation & proliferation GBV-C induces chemokines GBV-C CCR5 and CXCR4 on CD4 cells GBV-C replicates in T and B cells P=0.01 N=11 N=13 N=11 N=9 Study Design ns UIHC HIV Clinic Patients Invitation – Informed Consent Heparinized Blood/Serum Obtained CD4, HIV VL measured Serum => GBV-C VL Cells => flow cytometry Does GBV-C alter B cell & NK cell activation or alter monocyte CCR5/CXCR4 expression?

  2. P=0.018 P=0.009 B cell activation: GB+ < GB- p=0.024 HIV Viremic GB+ < GB- p=0.018 NK cell activation: CD56dim/CD16+: NS CD56brt/CD16-: If HIV RNA neg: NS If HIV RNA pos: GB+ < GB- p=0.009

  3. Conclusion: • GBV-C replication in vivo • Diminishes B cell activation • Blunts CD16+ NK cell activation • Downregulates monocyte CCR5 • GBV-C replicates in T and B cells; however, replication limited to a very small percentage of cells. • Replication has not been shown in NK cells or monocytes. Effect in these cells may be due to induction of chemokines and/or cytokines • The effect of GBV-C activation on T and B lymphocytes, NK cells and monocytes may contribute to prolonged survival in HIV- infected individuals. Monocyte CCR5 expression GB+ < GB- p=0.009 HIV RNA+: GB+ < GB- p=0.03 CXCR4 GB+< GB-, but NS P=0.03

More Related