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Interaction between Tetraspanins and ADAM10 in Cell Maturation Process

This study investigates the interaction of Tetraspanins CD9, Tspan12, and Tspan14 with ADAM10 to understand their role in promoting ADAM10 maturation. GFP-tagged mouse Tetraspanins were used in co-immunoprecipitation experiments, revealing different capacities to promote ADAM10 maturation. The results provide insights into the complex regulatory mechanisms of ADAM10 processing in cells.

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Interaction between Tetraspanins and ADAM10 in Cell Maturation Process

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  1. Tspan14 Tspan12 CD9 - GFP-Tspan: + + + + HA-ADAM10: A i.p: GFP-Tspan blot: HA-ADAM10 ** 125 80 80 100 HA-ADAM10 quantitation from GFP i.p. Relative ADAM10 level 75 58 58 58 50 25 0 B whole cell lysate blot: HA-ADAM10 ** 60 40 HA-ADAM10 quantitation from whole cell lysate % mature ADAM10 20 0 46 C 30 i.p: GFP-Tspan blot: GFP-Tspan 25 Supplementary Figure III.The non-TspanC8 tetraspanin Tspan12 does not interact with or promote ADAM10 maturation. The experiment was performed and presented as described in Figure 2A-C, with the exception that GFP-tagged mouse tetraspanins were used instead of FLAG-tagged tetraspanins. The GFP-tagged mouse forms of non-TspanC8 tetraspanins CD9 and Tspan12 were compared to Tspan14 in their capacity to (A) co-immunoprecipitate with, and (B) promote maturation of, bovine HA-tagged ADAM10 (n=5; **p<0.01 compared to all other data; error bars represent standard errors of the mean). The GFP immunoprecipitation was performed with GFP-Trap beads and confirmation of similar Tspan12 and Tspan14 expression was obtained by (C) anti-GFP western blotting.

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