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Supplementary Figure 1. A. TiO 2. 3500x. 8900x. B. Carbon black. 5600x. 14 000x. C. SWCNT. 1500x. 5000x. Supplementary Figure 2. P-2 κ B α immunostaining of WT and p53 KO macrophages. A. DMEM. SWCNT. p53 KO. WT. WT. p53 KO. Immunostaining quantification. B.
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Supplementary Figure 1 A TiO2 3500x 8900x B Carbon black 5600x 14 000x C SWCNT 1500x 5000x
Supplementary Figure 2 P-2κBαimmunostaining of WT and p53 KO macrophages A DMEM SWCNT p53 KO WT WT p53 KO Immunostaining quantification B Percentage of positively stained cells (%) P-IκBα DAPI Merge 2µg/cm2
Supplementary figure legends SuppFig 1.Transmission electron microscopy images of RAW 264.7 cells incubated with 10 µg/ml(2 µg/cm2) of manufactured nanomaterials (MNMs) for 6 hr. (A) RAW 264.7 cell with TiO2 A10 MNMs within a vesicle (B) CB P60 MNMs within the cytosolic compartment of a RAW cell. (C) RAW macrophages with SWCNT at the membrane surface. Supp Fig 2.Consequence of p53 activation: inflammatory response in primary wild-type (WT) and p53 knock-out (KO) macrophages. (A, B) The cells were exposed to 2 µg/cm2 of TiO2 (A10), CB (FW2) and SWCNT MNMs for 30 min. The data show the percentages of phosphorylated IκBα (P- IκBα) positively stained macrophages (mean ± SEM) (n=3). * significant difference from WT control (p < 0.05). # significant difference between WT and p53 KO macrophages (p < 0.05).
