1 / 11

Brief Review of DNA Replication:

Brief Review of DNA Replication:. Nearest Neighbor Analysis (Kornberg 1956):. In vitro DNA synthesis •Template DNA •DNA polymerase •dNTPs. *dATP. *dCTP. *dGTP. *dTTP. •Allow DNA replication to take place •Denature DNA •Treat with micrococcal nuclease-

arlene
Download Presentation

Brief Review of DNA Replication:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Brief Review of DNA Replication:

  2. Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis •Template DNA •DNA polymerase •dNTPs *dATP *dCTP *dGTP *dTTP •Allow DNA replication to take place •Denature DNA •Treat with micrococcal nuclease- yields mononucleotides with 3’ terminal phosphates

  3. ssDNA treated with micrococcal nuclease: A A C C C * G G T T T T C ssDNA: *

  4. Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) Which must be present in equal frequencies if the DNA strands are: Parallel ?Antiparallel?

  5. ApA ApC ApG ApT CpA CpC CpG CpT GpA GpC GpG GpT TpA TpC TpG TpT Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) Which must be present in equal frequencies if the DNA strands are: Parallel ?Antiparallel? ApC = TpG CpA = GpT ApG = TpC CpG = GpC ApT = TpA CpT = GpA ApC = GpT CpA = TpG ApG = CpT GpA = TpC

  6. Alternative Forms of DNA

  7. G + C Content

  8. DNA Melting *Temperature at which DNA strands are ½ denatured is the melting temperature or Tm *GC content of DNA has a significant effect on Tm

  9. Comparison of Typical Prokaryotic and Eukaryotic Cells

  10. Chromosome Sequence and Diversity

  11. Gene Density in Different Organisms

More Related