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The Neurochemistry of GALP: Where Form Meets Function

The Neurochemistry of GALP: Where Form Meets Function. Rachna Goswami Leo Reap. What is GALP?. Galanin-like peptide (GALP): neuropeptide; only expressed in a small number of neurons within the hypothalamus   GALP expression is drastically reduced during fasting, anorexia, and diabetes.

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The Neurochemistry of GALP: Where Form Meets Function

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  1. The Neurochemistry of GALP: Where Form Meets Function Rachna Goswami Leo Reap

  2. What is GALP? • Galanin-like peptide (GALP): neuropeptide; only expressed in a small number of neurons within the hypothalamus   • GALP expression is drastically reduced during fasting, anorexia, and diabetes. • GALP acts to alter food intake and metabolic rate, and it also functions to stimulate reproductive physiology and behaviors.  • The only two factors that regulate GALP are insulin and leptin.

  3. Through our research last summer on pre-pubertal rats we discovered…

  4. Rich N, Reyes P, Reap L, Goswami R, Fraley GS 2007 Sex differences in the effect of prepubertal GALP infusion on growth, metabolism and LH secretion. Physiology and Behavior in press

  5. Rich N, Reyes P, Reap L, Goswami R, Fraley GS 2007 Sex differences in the effect of prepubertal GALP infusion on growth, metabolism and LH secretion. Physiology and Behavior in press

  6. Rich N, Reyes P, Reap L, Goswami R, Fraley GS 2007 Sex differences in the effect of prepubertal GALP infusion on growth, metabolism and LH secretion. Physiology and Behavior in press

  7. Since GALP expression is the same in both males and females, these findings lead us to believe that the target sites for GALP neurons are sexually dimorphic.

  8. What are the projections of GALP through the brain?

  9. Phaseolus Vulgaris Arcuate Nucleus Neuronal Tract-Tracing GALP Sexually Dimorphic?

  10. Immunocytochemistry • Stain brain sections with antibody solutions • Assign each antibody a different color through the use of different fluorescent dyes • Using fluorescence microscope, we will be able to tell which neural fibers transport GALP and to which areas of the brain they project

  11. Summary Currently, we have: • Stereotaxically injected Phaseolus Vulgaris into rat brains • Processed brains 3) Successfully done preliminary ICC

  12. Acknowledgments Dr. Fraley HHMI NIH (K01 DK066238-01A1) REACH Hope College

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