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李玉军

Experiment 3. 李玉军. 化脓菌分离鉴定过程（三）. 化脓菌分离鉴定过程. 葡萄球菌 甘露醇发酵试验 血浆凝固酶试验 耐热核酸酶试验 动物试验. 甘露醇发酵试验. Inoculate the bacteria into a mannitol micro-tube Incubate at 37℃ for 18h. S.aureus will ferment mannitol to produce acid, which causes the medium to turn yellow. 纤维蛋白原. 协同因子.

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李玉军

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  1. Experiment 3 李玉军

  2. 化脓菌分离鉴定过程（三）

  3. 化脓菌分离鉴定过程 • 葡萄球菌 • 甘露醇发酵试验 • 血浆凝固酶试验 • 耐热核酸酶试验 • 动物试验

  4. 甘露醇发酵试验 • Inoculate the bacteria into a mannitol micro-tube • Incubate at 37℃ for 18h. • S.aureus will ferment mannitol to produce acid, which causes the medium to turn yellow.

  5. 纤维蛋白原 协同因子 血浆凝固酶试验（The Coagulase Test） • Coagulase • 结合凝固酶 • 游离凝固酶 • 凝固酶凝血酶样物质 纤维蛋白 • It has been speculated that this enzyme might be a virulence factor. However, coagulase-negative strains are often as pathogenic as coagulase-positive strains. • There are two ways of performing this test • The Slide Coagulase Test • The Tube coagulase Test

  6. The Slide Coagulase Test

  7. 细菌的药物敏感性试验 • 纸片扩散法（K-B法）

  8. 肠道菌分离鉴定过程（三）

  9. Antigens of salmonella O antigen polysaccharide of LPS used to divide Salmonella to 42 groups （A－Z，O51－63，O65－67） A-E groups are pathogenic H antigen flagella antigen divide Salmonella tow phase: special no special Vi antigen related to the virulence of Salmonella sensitive to heat ( lose activity at 60℃)

  10. Serological Identification of Salmonella Select the specimen React with A-Z polyvalent antiserum Agglutination reaction (＋) (－) React with several individual types of anti-O and anti-H antiserum React with anti-Vi antiserum Identify its group and phage

  11. 系统实验报告 肠道菌 化脓菌 目的 策略（原理设计） 方法步骤 结果（判定方法） 结论 讨论

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