Chapter 6 Ag-Ab Interactions - PowerPoint PPT Presentation

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Chapter 6 Ag-Ab Interactions

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Chapter 6 Ag-Ab Interactions
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Chapter 6 Ag-Ab Interactions

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  1. Chapter 6 Ag-Ab Interactions

  2. Nature of the Ag-Ab interaction Immunological assays

  3. 4 types of noncovalent forces

  4. Affinity The strength of the total noncovalent interactions between a single Ag-binding site on an Ab and a single epitope is the affinity of the Ab for that epitope. Avidity The strength of multiple interactions between a multivalent Ab and Ag is called the avidity. Example: secreted pentameric IgM often has a lower affinity than IgG, but the high avidity of IgM, resulting from its higher valence, enables it to bind Ag effectively.

  5. Immunological Assays 1. Precipitation 2. Agglutination 3. Radioimmunoassay 4. Enzyme-linked Immunosorbent Assay 5. Western Blotting 6. Immunofluorescence 7. Flow Cytometry and Fluorescence 8. Immunoelectron Microscopy

  6. Precipitation Reactions: Precipitation in fluids Precipitation in gels - radial immunodiffusion (Mancini method) - double immunodiffusion (Ouchterlony method) Immunoelectrophoresis

  7. Precipitation Reactions

  8. Lattice Formation (格子)

  9. Double Immunodiffusion (Ouchterlony method)

  10. Immunoelectrophoresis

  11. Agglutination Reactions: Hemagglutination Bacterial Agglutination Passive Agglutination Agglutination Inhibition

  12. Hemagglutination Agglutination No Agglutination No Ab

  13. Agglutination Inhibition

  14. Solid-phase Radioimmunoassay (RIA)

  15. Enzyme-linked Immunosorbent Assay (ELISA): - Indirect ELISA - Sandwich ELISA - Competitive ELISA - Chemiluminescence

  16. Elispot Assay

  17. Western Blotting

  18. Immunofluorescence

  19. Flow Cytometry FACS: Fluoresence-activated Cell sorter

  20. Immunoelectron microscopy