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Anacyte Laboratories GmbH

Anacyte Laboratories is dedicated to facilitate and improve molecular analyses of biomolecules.

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Anacyte Laboratories GmbH

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  1. CELLCOVER APPLICATION GUIDE FLOW CYTOMETRY & IMMUNOFLUORESCENCE PDF processed with CutePDF evaluation edition www.CutePDF.com PDF processed with CutePDF evaluation edition www.CutePDF.com

  2. FLOW CYTOMETRY AND IMUNNOFLUORESCENCE WITHCELLCOVER CellCoverisa new and unique reagent allowing sophisticated immuno-analyseslike flow cytometry or immuno-localisation of proteins. CellCovers liquid freeze effect preservesRNA and protein stability and keepsmoleculesin place which isespecially usefulforanalysing proteinsinsitu. CellCover allows storage of cells. Cells treated maintain morphology for several days. Epitopes are protected and can be stained later. CellCover can also be used to stabilize the immuno­staining of cells, if later analysis is required. with CellCover Unique feature of CellCover is the possibility to isolate highly intact RNA with high RIN values from cells in which protein has already been stained by immuno­labelling. Quality of RNA afterCellCoverprotection: Cells have been immunostained for protein, RNA was isolatedsubsequently. CellCovers liquid freeze effect allows visualization of formalin sensitive epitopes like Vimentin, enabling new insights in cells structure and architecture. DISCOVER MORE WITHCELLCOVER

  3. FLOW CYTOMETRY AND IMUNNOFLUORESCENCE APPLICATION CellCoverexertsitsstabilizing effect very fast.DNA,RNA,protein stay in place in a close to native condition, without crosslinking activity! Harsh treatments with alcoholoracetonearenot longerobligateinanalysisofcells. IMMUNO-LOCALISATION OF PROTEIN IN CELLS PREPARING CELLS FOR FLOW CYTOMETRIC ANALYSIS AND CELL SORTING 1.seedandgrow cells 2.removemedium 3.placeslideinCellCover 1.harvest andpellet cells 2.removesupernatant 3.resuspendcellsinCellCover Note: Attachment ofcellstothesubstrate is critical.CellCoverdoesnot havecrosslinking properities. Ifyouexperiencetomanycells floating offthesubstrate,youcantryfollowing workarounds: -coat substratewithpoly-lysin -dipinPBS,drainslideanddrysample -oracombinationofboth Storeat 4°Cuntiluse 4.pellet cellsandremoveSN 5.permeabilizecellswithPBS/T,(e.g.15min) 6.immuno-staincellsasrequired Note: IfRNA istobeisolatedlaterstaining procedureshouldbecarriedout asfast as possible. StoreinCellCoverat 4°Cuntiluse 7.Counterstainifnecessary 5.diponceinPBS 6.proceedtostandardimmunofluorescence staining protocols StorecellsinCellCoveruntiluse. 8.Proceedtoflow cytometricanalyis. Note: Most antigenscanbevisualizedwithout pretreatment likecooking inCitricAcid, however,staining might beintensifiedbyit. Pretreatment must beverygentle,avoid boiling orothershearstress.Cellsmust be handledverycarefullythroughout thewhole procedure. Note:Ifsubpopulationsofcellsshallbe collectedcellsarebest sortedintoCellCover.

  4. www.anacyte.com YOUR LOCALDISTRIBUTOR Sambo Medical Co. Tel.02-575-4945,Fax.02-574-7595 www.sambomed.co.kr info@sambomed.co.kr AL Anacyte Laboratories UG / Classenstieg 23 / 22393 Hamburg / Germany / www.anacyte.com / info@anacyte.com

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