2012 년 정리

1. 2012년 정리 화순전남대학교병원 미토콘드리아 연구실 (Molecular Diagnostics and       Mitochondrial Research Laboratory) 홈페이지- http://www.mtdna.or.kr 2012. 12. 28 (금)

2. Molecular Diagnostics and Mitochondrial research laboratory Nucleus cell Hematologic Malignancy Hematopoietic Stem cells Treatment Outcome MRD determination Biomarkers Aging: HSC, BM Environment New molecular Pathophysiology Potential new therapeutics Solid Organ Cancer Diagnosis Chronic Inflammatory/ Degenerative Diseases

3. 정리 내용 • 연구원 동정 • 학술대회 발표 및 초록제출 • Accepted manuscript • 준비중인 SCI논문 • 연구과제 수행

4. 2012년 MTDNA연구팀실험실 동정 현재 신명근 교수님, 김혜란, 김환영, Trang(창) 연구원 구성 • 2012. 03. 1 김환영, Nguyen Thi Dai Trang 전남대하교 의과대학 분자의과학 협동과정 입학 • 2012. 02.08 2012 대한진단혈액학회 학술대회 (서울대학교 어린이병원) -최우수연제상 • 2012.04.28 KMMWP 1st Consensus Conference&2nd Research Meeting (화순전남대학교병원 대강당) 개최 • 2012. 05. 21~24 국제 진단혈액학회(ISLH) 참석 ( France, Nice) • 2012. 06. 01~02 대한혈액학회 참석 (서울그랜드힐튼 호텔 ) • 2012. 10. 06 MPN/CML Symposium 2012 (세브란스병원) • 2012. 10. 06 The 2nd Korea-Germany International Joint Symposium (화순전대병원) • 2012. 10. 13 AML/MDS 연구회 추계 심포지엄 (화순전남대학교병원) • 2012. 10. 15~16 대한진단검사의학회 제 53차 학술대회참석 (서울그랜드힐튼 호텔) • 2012. 10. 19 화순전남대병원 임상의학연구소 Wet-Bench Workshop • 2012. 11. 12~13 The GRDC(Global R&D Centers) Symposium참석(해외협력유치사업) • 2012. 11. 16~17 환경보건센터 워크샵 참석 (강원도 횡성) • 2012. 12. 07~14 미국혈액학회(ASH, Atlanta, GA) 참석-신명근교수님, 김환영, Trang • 2012. 12. 12~16 TRM-Award 2012(University LEIPZIG in Germany) 참석-김혜란 박사님

5. 2012 학술대회 발표 및 초록제출 1) 2012 대한진단혈액학회 학술대회(2012.02.08), 서울대학교 어린이병원 - Multiple Myeloma Panel: Dx Criteria, Risk Stratification&Response Criteria - New potential chemotherapeutic agents targeting prohibtib selectively killed AML cells in a mouse AML model (최우수연제상 수상) - Benzopyrene exposure induced increase of mitochondrial mass and mitochondrial DNA copy number in blood cells and hematopoietic stem cells 2) 제 21차 대한 노인병학회 호남지회 연수강좌 (2012.02.11), 전남대학교 의과대학 덕재홀 - 조기 암 스크리닝 최신 기법 3) 2012 Hwasun International Symposium on Genomics and Cell Therapy Joint Commemorative Symposium of Prof.Hwang's Retirement by KSPHO(2012.02.25), 광주 김대중 컨벤션 센터 - Recent Studies and Reports of Molecular Diagnostics in CNUHH Laboratory Medicine 4) KHUPO 12th Annual International Proteomics Conference (2012.03.29),서울 대학교 - Use of MDLC-DIGE and LC-MS/MS to identify serum biomarkers for complete remission in patients with acute myeloid leukemia 5) KMMWP 1st Consensus Conference&2nd Research Meeting(2012.04.28), 화순전남대학교병원 대강당 - Diagnostic panel & follow up panel for MM

6. 6) ISLH XXV International Symposium on Technical Innovations in Laboratory Hematology (2012.05.21-24), Nice, France - Prohibitin alkylating agents as new potential chemitherapeutics selectively inhibited AML cells in a mouse AML model - Mitochondrial DNA markers were superior to nuclear DNA Markers for the quantitative detection of chimerism, particularly in unrelated allogeneic stem sell transplantation - Benzopyrene exposure induced increase of mitochondrial mass and mitochondrial DNA copy number in leukemia and blood cells 7) The 53rd spring meeting of the Korean Society of Hematology(2012.06.01-02) 서울그랜드 힐튼 컨벤션 센터 - DNI and LUC as New CBC parameters can discriminate acute proelocytic leukemia cells form other myeloid leukemia cells and cells at remission status - Mitochondrial DNA copy number associated with risk of childhood Acute Myeloid Leukemia - The pronostic implication of parathyroid hormone(PTH) in Multiple Myeloma Patients - Revisited Prognostic impact of NPM1, BAALC, WT1 and FLT3 Mutation in Korean patients with de Novo Acute Myeloid Leukemia - Ex Vivo Expansion of Human Natural Killer cells using Cryopreserved Irradiated Feeder cells - Remarkable Decreased Expression of Notch3, FGFR1, FZD5, GLI1 and FZD5 Maintained Quiescent Property of AML stem cells

7. 8) Hwasun Biomedical Workshop 2012 (2012.07.20), 화순전남대학교병원 대강당 - Recurrent spliceosome mutations in hematological malignancies and their clinical implications - Marked decreased expression of Notch3, FGFR1, FZD5,GLI1 and FZD5 gene contributed to quiescent property of AML stem cells 9) The 13th KOREA-JAPAN-CHINA COLORECTAL CANCER SYMPOSIUM (2012.09.08), Seoul - Her-2 expression status from serum and cancer tissue in patients with colorectal cancer 10) The 2nd Korea-Germany international Joint Symposium(2012.10.06), 화순전남대학교병원 - Aging-related changes of hematopoietic stem cells 11) MPN/CML Symposium 2012 (2012.10.06), 세브란스병원 - Diagnosis of MDS/MPN 12) 제11회 대한혈액학회 AML/MDS 연구회 추계 심포지엄 (2012.10.13), 화순전남대학교병원 - New insights in molecular mechanisms of MDS and clinical implication 13) 53rd Annual Meeting of the Korean Society for Laboratory Medicine (2012.10.15-16) Grand Hilton Seoul Hotel - Recurrent Spliceosome Mutation in Other Hematological Disorders and Perspectives (진단혈액 심포지엄 강의) - Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for the Identification of Candida Bloodstream Isolates Recovered from 10 Korean Hospitals over a 1-year Period - Clinical Implication of SF3B1 and U2AF1 Spliceosomal Gene Aberrations in Korean Multiple Myeloma Patients

8. - New Biomarkers and Biological Characteristics of Polycyclic Aromatic Hydrocarbon Exposure in an Ex Vivo Cell Line Model - Diagnostic Value of Serum Protein Induced cy Vitamin K Absence of Antagonist-II in Various Malignancies except Hepatocellular Carcinoma - Clinicopathological Characteristics of Acute Leukemia with MLL/MLLT4 Gene Rearrangement - Usefulness of Plasma Epigenetic Changes in Five Major Genes Involved in the Pathogenesis of Colorectal Cancer - Changing Distribution of Yeast Species Isolated from Sterile and Nonsterile Sites in Korea - Diagnostic Value of Direct Serum Immunoglobulin Heavy/Light Chain Measurement in Ambiguous Electrophoresis Patterns - Urine Sediment as a Suitable Specimen for Quantitative PCR Assay of Cytomegalovirus - Emergence of Colistin-Resistant Bloodstream Isolates of Acinetobacter Genomic Species 13BJ in a University Hospital - Cytokine Profiles Provide Distinct Signatures for Different Stages of Tuberculosis is Korea - Effects of Exposure to IL-21 at Different Time Points on the Ex Vivo Expansion of Natural Killer Cells from Human Peripheral Blood - The Cis-AB01 Allele Originated from the A105 Allele

9. 14) AMP 2012 Annual Meeting on Genomic Medicine (2012.10.26), Long Beach, LA, CA, USA - Comparison of whole-blood IGRA, TST, PCR, and HRCT for rapid diagnosis of smear-negative pulmonary tuberculosis in Korea 15) 54th Annual Meeting of The American Society of Hematology(2012.12.07-13), Atlanta,GA - No Recurrent Mutation of SF3B1, U2AF1 and SRSF2 Spliceosomal Gene in Multiple Myeloma but Polymorphisms of These Genes Are Associated with the Prediction of Worse Prognosis - Elevation of mitochondrial DNA copy number in bone marrow cells associated with increased risk of childhood acute myeloid leukemia 16) TRM-Award 2012(2012.12.14),University LEIPZIG in Germany - Discovery of prognostic mitochondrial markers for leukemia cells and leukemia stem cells

10. 2012 ACCEPTED MANUSCRIPT • 주저자 및 교신저자 • Paroxysmal nocturnal hemoglobinuria-myelodysplastic syndrome associated with marked thrombocytosis and acquired clonal cytogenetic abnormality of t(3;12)(q28;q15). Leuk Lymphoma. 2012 Jul;53(7):1429-32. • HLA-A*02:07:02, a new allele identified by sequence-based typing in a Korean individual. Tissue Antigens. 2012 May;79(5):384-5. • Frequent Occurrence of Mitochondrial DNA Mutations in Barrett's Metaplasia without the Presence of Dysplasia. PLoS One. 2012;7(5):e37571. • Usefulness of plasma epigenetic changes of five major genes involved in the pathogenesis of colorectal cancer. Int J Colorectal Dis. 2012 Sep 19. [Epub ahead of print] • A new HLA-C*07:244 allele identified by sequence-based typing in a Korean individual. Tissue Antigens. 2012 Oct;80(4):384-5. • Association of allelic expression imbalance of JAK2 V617F mutation • and phenotypic diversity in MPN: In press PLoS One • Overexpression of Her-2 in colorectal cancer tissue, but not in serum, constitutes an independent worse prognostic factor: Major revision Cellular Oncology • Mitochondrial DNA minisatellites markers had multi-functions for monitoring mixed chimerism and predicting clinical outcome after stem cell transplantation : Submitted American Jounal of Hematology

11. 공저자 • Unicentric Castleman Disease Relapsed After Rituximab-CHOP Chemotherapy or Radiation Therapy in an Adolescent. J Pediatr Hematol Oncol. 2012 Jul;34(5):e206-8. • An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells. Ann Clin Lab Sci. 2012 Winter;42(1):42-9. • Detection of HTLV-1 in the Labial Salivary Glands of Patients with Sjogren's Syndrome: A Distinct Clinical Subgroup? J Rheumatol. 2012 Apr;39(4):809-15. • Interleukin-21 increases direct cytotoxicity and IFN-γ production of ex vivo expanded NK cells towards breast cancer cells. Anticancer Res. 2012 Mar;32(3):839-46. • Dysfunction of Natural Killer T Cells in Patients with Active Mycobacterium tuberculosis Infection. Infect Immun. 2012 Jun;80(6):2100-8. • Detection of Amphotericin B Resistance in Candida haemulonii and Closely-Related Species Using the Etest, Vitek-2 Yeast Susceptibility System, and CLSI and EUCAST Broth Microdilution Methods. J Clin Microbiol. 2012 Jun;50(6):1852-5.

12. 공저자 • Complete Sequencing of the ABO Alleles of the Common ABO Group and Rare ABO Subgroups in Koreans. Korean J Blood Transfus. 2012 Apr;23(1):1-12. • Ex vivo Expansion of Human Natural Killer Cells from Blood Retained in a Disposable Platelet Apheresis Set. Korean J Blood Transfus. 2012 Apr;23(1):28-37. • Scrub Typhus-associated Severe Hemophagocytic Lymphohistiocytosis With Encephalomyelitis Leading to Permanent Sequelae: A Case Report and Review of the Literature. J Pediatr Hematol Oncol. 2012 May 22. [Epub ahead of print] • Validation of trans-acting elements that promote exon 7 skipping of SMN2 in SMN2-GFP stable cell line. Biochem Biophys Res Commun. 2012 Jun 7. [Epub ahead of print] • Use of MDLC-DIGE and LC-MS/MS to identify serum biomarkers for complete remission in patients with acute myeloid leukemia. Electrophoresis. 2012 Jul;33(12):1863-72. • Age- and gender-related differences in circulating natural killer T cells and their subset levels in healthy Korean adults. Hum Immunol. 2012 Oct;73(10):1011-6.

13. 공저자 • A genome-wide single-nucleotide polymorphism-array can improve the prognostic stratification of the core binding factor acute myeloid leukemia. Am J Hematol. 2012 Oct;87(10):961-8. • Molecular identification of Schizophyllum commune as a cause of allergic fungal sinusitis. Ann Lab Med. 2012 Sep;32(5):375-9. • Associations of BDNF genotype and promoter methylation with acute and long-term stroke outcomes in an East asian cohort. PLoS One. 2012;7(12):e51280.

14. 2012 Manuscript preparation Prohibitin as a new biomarkers of leukemia for diagnosis, predicting prognosis, MRD monitoring and molecular therapeutics The spectrum of mitochondrial genome instability in acute myeloid leukemia and leukemia stem cells The change of mtDNA sequence aberration, mtDNA content/mass, mtDNA copy number and clinical values in childhood leukemia Clinical significance of mtDNA copy number in comparison with samples at Dx and F/U (initial remission or relapse) Serum fibrin degradation product is a valuable tumor marker for multiple myeloma Mitochondrial minisatellite alterations in single AML stem cells associated with vicious cycle hypothesis  Accuracy improvement for glucometer by reducing the hematocrit dependency using informatics technology Mitochondrial Biomarkers for Benzopyrene Exposure

15. 2012 연구과제 수행 • 한국연구재단 중견연구자사업 • 한국연구재단 해외우수연구소유치사업 • 한국연구재단 일반연구자사업 • 환경성 질환연구센터 3세부과제

16. 환경보건센터 워크샵 (강원도 횡성) 2012 ASH (Atlanta), CNN 센터, Coca-cola 본사에서~

17. 크리스마스마켓 파우스트 동상 게반트하우스 TRM-Award 2012(University LEIPZIG in Germany)