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Learn how cells transform energy through processes like cellular respiration, the role of ATP as an energy currency, and the significance of phosphorylation in releasing and transferring energy within organisms.
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Carbon Cycles Through Organisms autotrophes heterotrophes
All Cells Respire Plants don’t do photosynthesis FOR animals They metabolize the sugars themselves!
Respiration Transforms Energy Anaerobically (without O2): 4 ATP Aerobically: 38 ATP It’s catabolic! Its exergonic! It’s redox! It’s enzyme-facilitated!
Recall: Catabolic & Exergonic - breaking down & releasing energy Redox - transferring e- Enzyme facilitated glucose oxygen releasing nrg forming water
Fuel the Body to fuel the cells 1. Consume organic molecules • carbohydrates, lipids, proteins 2. Digest them, releasing energy • catabolic rxn 3. Cells transform released energy into a useable form; an energy currency • a vehicle to pass energy around • a short term energy storage molecule ATP
ATP -Adenosine TriPhosphate • A modified nucleotide adenine + ribose + PO4- AMP AMP + PO4- ADP ADP + PO4- ATP • • adding the P is key! • phosphorylation
O– O– O– O– O– O– O– O– P P P P P P P P –O –O –O O– O– O– –O –O –O O– O– O– –O –O O– O– O O O O O O O O ATP stores energy ~ Each PO4- more difficult to add • negative to negative • a lot of stored energy in each bond (most in the third) AMP ADP ATP • ~ Final Pgroup pops off easily & transfers energy • • bonding of Pgroups is unstable • • instability makes ATP a great energy donor
O– O– O– O– P P P P –O O– –O O– –O –O O– O– O O O O Phosphorylation Transfers Energy • ATP ADP : Releases energy • Use to fuel other reactions : Phosphorylation • released PO4- transferred to another molecule destabilizes the other molecule by stealing e- • phosphorylation facilitated by enzyme kinase 7.3 kcal + ADP ATP
+ P ATP / ADP are cycled ATP • ATP is unstable ~ good energy donor ~ poor energy storage too reactive; transfers P easily ~ a renewable resource ADP A working muscle recycles over 10 million ATPs per second
P ATP C 2 hexokinase C 2 ADP phosphofructokinase H C P Phosphorylation Occurs in Glycolysis Phosphate transfer activates the breakdown of glucose glucose C-C-C-C-C-C P-C-C-C-C-C-C-P P-C-C-C C-C-C-P PGAL - Phosphoglyceraldehyde….. becoming Pyruvate
2 ATP Glycolysis ‘sugar’ + ‘break apart’ Occurs in cytoplasm (cytosol) of all organisms 10 step biochemical pathway Enzyme - facilitated Reactant: 6 C glucose + 2 ATP Product: two 3C pyruvate +4 ATP + 2 NADH e- e- NADH NADH?
4 ATP Start here Got fructose? Enter pathway here Invest 2 ATP phosphorylate PGAL e- e- 2 NADH Again, NADH??
Electron Transfer Molecules *NAD+ coenzymes NADH ^FAD cofactors FADH2 oxidizingreducing donates e-accepts e- *Nicotinamide adenine dinucleotide ^Flavin adenine dinucleotide
Pyruvate options Anaerobic options All Organisms plants & animals yeast bacteria muscle cells
Anaerobic Resp v1 : alcoholic fermentation 3 C Pyruvate acetlyaldehyde + CO2 2 C ethanol
Anaerobic Resp v1 : alcoholic fermentation Ethanol is toxic to yeast at about 12% limiting the alcohol content of naturally fermented products some bacteria & yeast
Fermentation v2: Lactate 3 C Pyruvate 3 C lactate (lactic acid) (No CO2)