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AtNAP, a NAC family trancription factor, has an important role in leaf senescence

学术报告 — 文献阅读汇报. AtNAP, a NAC family trancription factor, has an important role in leaf senescence. 报 告 人：王华 专业年级：食品科学 2010 级 指导老师：薛照辉副教授. Result and Conclusion. Introduction. Discussion. 1. 2. 3. 4. Outline. Experiment content. TF.

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AtNAP, a NAC family trancription factor, has an important role in leaf senescence

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  1. 学术报告—文献阅读汇报 AtNAP, a NAC family trancription factor,has an important role in leaf senescence 报 告 人：王华 专业年级：食品科学2010级 指导老师：薛照辉副教授

  2. Result and Conclusion Introduction Discussion 1 2 3 4 Outline Experiment content Company Logo

  3. TF 能够结合在某基因上游特异核苷酸序列上的蛋白质，活化后从胞质转位至胞核，通过识别和结合基因启动子区的顺式作用元件,启动和调控基因表达。 Introduction 1.1 Leaf Senescence genes Transcription Factors

  4. DREB bZIP WRKY NAC MYB Introduction 1.2 NAC family transcription factor NAM 植物生长发育 NAC ATAF1/2 激素调节 CUC2 环境胁迫应答 含有NAC结构域的蛋白统称为NAC蛋白

  5. Experiment content Inducible overexp- ression of AtNAP AtNAP expression in T-DNA line Subcellular location of AtNAP AtNAP expression during leaf senescence

  6. Results and Conclusion 3.1AtNAP is upregulated during leaf sencence in Arabidopsis

  7. Results and Conclusion 3.2AtNAP is targeted to nuclei

  8. Results and Conclusion 3.3The AtNAP expression is knocked out in one T-DNA line and knocked down in another line

  9. Results and Conclusion 3.4Leaf senescence is significantly delayed in the atnap null mutant plants

  10. Results and Conclusion

  11. Results and Conclusion

  12. Results and Conclusion 3.5 AtNAP homologs in rice and kidney bean are specifically expressed in senescing leaves

  13. Results and Conclusion

  14. Results and Conclusion 3.6AtNAP restores the atnap null mutant plants to wild type Company Logo

  15. Results and Conclusion 3.7Inducible overexpression of AtNAP causes precious senescence

  16. Results and Conclusion

  17. Results and Conclusion 3.8Microarray analysis of the expression profile of AtNAP

  18. AtNAP 与叶的衰老有关 T-DNA插入失活AtNAP叶衰老延迟 超量表达AtNAP基因造成早熟性衰老 Disscussion RT-PCR RNA gel blot T-DNA insertions Plasmid construction Plant transformation Complementation test

  19. Refferences 1.冶晓芳,唐益苗,高世庆,等.植物NAC转录因子的研究进展. 生物计数通报,2009,10(6)20–24. 2.李鹏,黄耿青,李学宝.植物NAC转录因子. 植物生理与分子生物学,2010,46(3)294–300. 3.郭玉娟,魏强.高羊茅中衰老相关转录因子AtNAP同源基因的克隆及功能分析. 复旦学报,2010,49(5)544–558. 4.彭辉,于兴旺,成慧颖,等.植物NAC转录因子家族研究概况. 植物学报,2011,45(2)236–248.报,2011,45(2)236–248. 5.史红飞,高翔,陈其皎, 等.小麦NAC转录因子的基因克隆与序列分析. 西北农林科技大学学报,2011,31(3)395–401. 6.易素华,张 丞,史志浩,等.拟南芥花粉表面蛋白基因T-DNA插入突变体的鉴定.上海师范大学学报,2010,39(2)313–318. 7.尤扬,叶永忠,等.植物激素对黄姜叶片Fv/Fo和Fv/Fm的影响.安徽农业科学,2007,35(19)5707–5703,5744.

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