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Garlic and Health, P9 report. Liverpool, 17-21 February 2003 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician F. Mirrione, temporary agent (1.5 month). Objectives. Somatic embryogenesis, genetic characterisation plant regeneration

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Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Garlic and Health, P9 report

Liverpool, 17-21 February 2003

  • INRA, Avignon, France

    • V. Chovelon, research scientist

    • JP Leroux, research technician

    • F. Mirrione, temporary agent (1.5 month)


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Objectives

  • Somatic embryogenesis, genetic characterisation

    • plant regeneration

    • acclimatisation and field evaluation

  • sanitary aspects

    • meristem culture on selected clones

    • production of virus-free bulbs


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Milestones

  • Embryogenesis


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Milestones

• Sanitary aspects



Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Rouge Reunion

  • plants from callus and cell suspensions

  • In vitro culture (1)

  • acclimatisation (2)

    • AFLP analyses

    • flow cytometry

    • phenotypic notations

- 1st field evaluation at Reunion Island by P8 (3)


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Messidrôme and Morasol

plants from callus or cell suspensions

• In vitro culture (2)

- regeneration

- bulb maturation

• Acclimatisation (2)

• 1st field culture (3) (4)

166 Mes cal; 145 Mol cal; 125 Mol ecs;153 Mes ecs

• 2nd field culture(4)

- 282 Messidrôme cal

- 143 Morasol cal

- 145 Morasol ecs


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

1st field evaluation

  • notations (plant development, height, leaves colour, bulbs shape and weight)

  • ploïdy level


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

1st field evaluation

• sulphur compounds concentration (HPLC)

Morasol and Messidrôme

Rouge Reunion


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

1st field evaluation

• sulphur compounds proportion (HPLC)

Morasol and Messidrôme

Rouge Reunion


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Printanor

plants from callus


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Sanitary aspects

  • production of virus-free plants

    • Messidrôme, Morasol, Printanor

    • 300 plants / var. (Nov-Dec 02)

  • virological control

    • all the plants were potyvirus-free

    • some one contained latent viruses

      • Messidrôme: 100% GCLV

      • Morasol: 83% MbFV

      • Printanor: 100% MbFV


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Sanitary aspects

  • virus elimination by meristem culture


Liverpool 17 21 february 2003 inra avignon france v chovelon research scientist

Workplan for 2003

  • Embryogenesis, genetic characterisation

    • Rouge Reunion

      • 2nd field evaluation (P8), biochemistry (P5)

    • Messidrôme, Morasol and Printanor from callus

      • 2nd field culture and evaluation (P9)

      • flow cytometry (P8), AFLP (P1), biochemistry (P5)

    • Messidrôme, Morasol and Printanor from cell suspension

      • 1st field evaluation

      • flow cytometry (P8), AFLP (P1), biochemistry (P5)

  • Sanitary aspects

    • production of virus-free bulbs (P9)