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17.2, 679 Pink box is what is in common, squiggly blue line is mature protein

17.2, 679 Pink box is what is in common, squiggly blue line is mature protein. 17.4, 681 Importing proteins into mitochondria translated protein binds to HSPs MSF or HSC70 2) MSF binds to Tom 70,37 (membrane-bound receptors) then pass off to Tom 20,22 3) HSC delivered direct

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17.2, 679 Pink box is what is in common, squiggly blue line is mature protein

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  1. 17.2, 679 Pink box is what is in common, squiggly blue line is mature protein

  2. 17.4, 681 • Importing proteins into • mitochondria • translated protein binds • to HSPs MSF or HSC70 • 2) MSF binds to Tom 70,37 • (membrane-bound receptors) • then pass off to Tom 20,22 • 3) HSC delivered direct • to Tom 20,22 • 4) Through outer with • Tom40 • 5) Through inner with • Tims-requires proton • gradient • 6) protein released, • target cleaved off, • folds with or without • HSPs

  3. 17.5, 683 Experiments to figure out how mito import works DHPR normally cytoplasmic-import into mitos if you add tag and spacer-won’t work if it can’t unfold or if spacer is too short It has to cross “contact site” between outer and inner membrane.

  4. 17.28, 710 Cell can detect unfolded proteins and responds by making HSPs 1RE membrane bound inner nuclear membrane- continuous with er When it detects unfolded proteins, dimerizes, causes mRNA for transcription factor to be processed, and this transcription factor causes HSPs to be transcribed

  5. 17.29, 711 How do things that reside in ER stay there? KDEL receptors- Lysine, Aspartate, Glutamate, Leucine But KDEL receptor itself is not a KDEL protein (!)

  6. 17.40, 721 getting lysosomal proteins to lysosomes without killing everybody along the way

  7. 17.45, 46 Import of cholesterol into cell

  8. New Stuff: Keller et al., 2001 Nature Cell Biology 3:140 Multicolour imaging of post-Golgi sorting and trafficking in liver cells Go to Quicktime movies

  9. Group Exercise 8 • Familial hypercholesterolemia is characterized by high • cholesterol levels within families, that may or may not be • related to diet. Speculate as to what is the cause of this. • 2) V-type proton pumps are similar in structure to parts of • the F1F0 complex in mitochondria, and they work to acidify • vesicles inside cells. If you have a mutation in which these • don’t work well, what can’t you do? (may be a list of many things)

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